Cargando…

Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy

Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct...

Descripción completa

Detalles Bibliográficos
Autores principales: Filenko, Nina A., Palets, Dmytro B., Lyubchenko, Yuri L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268019/
https://www.ncbi.nlm.nih.gov/pubmed/22312477
http://dx.doi.org/10.1155/2012/650840
_version_ 1782222340059299840
author Filenko, Nina A.
Palets, Dmytro B.
Lyubchenko, Yuri L.
author_facet Filenko, Nina A.
Palets, Dmytro B.
Lyubchenko, Yuri L.
author_sort Filenko, Nina A.
collection PubMed
description Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.
format Online
Article
Text
id pubmed-3268019
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-32680192012-02-06 Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy Filenko, Nina A. Palets, Dmytro B. Lyubchenko, Yuri L. J Amino Acids Research Article Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed. Hindawi Publishing Corporation 2012 2011-10-23 /pmc/articles/PMC3268019/ /pubmed/22312477 http://dx.doi.org/10.1155/2012/650840 Text en Copyright © 2012 Nina A. Filenko et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Filenko, Nina A.
Palets, Dmytro B.
Lyubchenko, Yuri L.
Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title_full Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title_fullStr Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title_full_unstemmed Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title_short Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
title_sort structure and dynamics of dinucleosomes assessed by atomic force microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268019/
https://www.ncbi.nlm.nih.gov/pubmed/22312477
http://dx.doi.org/10.1155/2012/650840
work_keys_str_mv AT filenkoninaa structureanddynamicsofdinucleosomesassessedbyatomicforcemicroscopy
AT paletsdmytrob structureanddynamicsofdinucleosomesassessedbyatomicforcemicroscopy
AT lyubchenkoyuril structureanddynamicsofdinucleosomesassessedbyatomicforcemicroscopy