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Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268019/ https://www.ncbi.nlm.nih.gov/pubmed/22312477 http://dx.doi.org/10.1155/2012/650840 |
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author | Filenko, Nina A. Palets, Dmytro B. Lyubchenko, Yuri L. |
author_facet | Filenko, Nina A. Palets, Dmytro B. Lyubchenko, Yuri L. |
author_sort | Filenko, Nina A. |
collection | PubMed |
description | Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed. |
format | Online Article Text |
id | pubmed-3268019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32680192012-02-06 Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy Filenko, Nina A. Palets, Dmytro B. Lyubchenko, Yuri L. J Amino Acids Research Article Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed. Hindawi Publishing Corporation 2012 2011-10-23 /pmc/articles/PMC3268019/ /pubmed/22312477 http://dx.doi.org/10.1155/2012/650840 Text en Copyright © 2012 Nina A. Filenko et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Filenko, Nina A. Palets, Dmytro B. Lyubchenko, Yuri L. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title | Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title_full | Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title_fullStr | Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title_full_unstemmed | Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title_short | Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy |
title_sort | structure and dynamics of dinucleosomes assessed by atomic force microscopy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268019/ https://www.ncbi.nlm.nih.gov/pubmed/22312477 http://dx.doi.org/10.1155/2012/650840 |
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