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Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice

Dietary supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFAs), specifically the fatty acids docosahexaenoic acid (DHA; 22:6 ω-3) and eicosapentaenoic acid (EPA; 20:5 ω-3), is known to have beneficial health effects including improvements in glucose and lipid homeostasis and modulation of...

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Autores principales: Burri, Lena, Berge, Kjetil, Wibrand, Karin, Berge, Rolf K., Barger, Jamie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268598/
https://www.ncbi.nlm.nih.gov/pubmed/22303341
http://dx.doi.org/10.3389/fgene.2011.00045
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author Burri, Lena
Berge, Kjetil
Wibrand, Karin
Berge, Rolf K.
Barger, Jamie L.
author_facet Burri, Lena
Berge, Kjetil
Wibrand, Karin
Berge, Rolf K.
Barger, Jamie L.
author_sort Burri, Lena
collection PubMed
description Dietary supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFAs), specifically the fatty acids docosahexaenoic acid (DHA; 22:6 ω-3) and eicosapentaenoic acid (EPA; 20:5 ω-3), is known to have beneficial health effects including improvements in glucose and lipid homeostasis and modulation of inflammation. To evaluate the efficacy of two different sources of ω-3 PUFAs, we performed gene expression profiling in the liver of mice fed diets supplemented with either fish oil (FO) or krill oil (KO). We found that ω-3 PUFA supplements derived from a phospholipid krill fraction (KO) downregulated the activity of pathways involved in hepatic glucose production as well as lipid and cholesterol synthesis. The data also suggested that KO-supplementation increases the activity of the mitochondrial respiratory chain. Surprisingly, an equimolar dose of EPA and DHA derived from FO modulated fewer pathways than a KO-supplemented diet and did not modulate key metabolic pathways regulated by KO, including glucose metabolism, lipid metabolism and the mitochondrial respiratory chain. Moreover, FO upregulated the cholesterol synthesis pathway, which was the opposite effect of krill-supplementation. Neither diet elicited changes in plasma levels of lipids, glucose, or insulin, probably because the mice used in this study were young and were fed a low-fat diet. Further studies of KO-supplementation using animal models of metabolic disorders and/or diets with a higher level of fat may be required to observe these effects.
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spelling pubmed-32685982012-02-02 Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice Burri, Lena Berge, Kjetil Wibrand, Karin Berge, Rolf K. Barger, Jamie L. Front Genet Genetics Dietary supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFAs), specifically the fatty acids docosahexaenoic acid (DHA; 22:6 ω-3) and eicosapentaenoic acid (EPA; 20:5 ω-3), is known to have beneficial health effects including improvements in glucose and lipid homeostasis and modulation of inflammation. To evaluate the efficacy of two different sources of ω-3 PUFAs, we performed gene expression profiling in the liver of mice fed diets supplemented with either fish oil (FO) or krill oil (KO). We found that ω-3 PUFA supplements derived from a phospholipid krill fraction (KO) downregulated the activity of pathways involved in hepatic glucose production as well as lipid and cholesterol synthesis. The data also suggested that KO-supplementation increases the activity of the mitochondrial respiratory chain. Surprisingly, an equimolar dose of EPA and DHA derived from FO modulated fewer pathways than a KO-supplemented diet and did not modulate key metabolic pathways regulated by KO, including glucose metabolism, lipid metabolism and the mitochondrial respiratory chain. Moreover, FO upregulated the cholesterol synthesis pathway, which was the opposite effect of krill-supplementation. Neither diet elicited changes in plasma levels of lipids, glucose, or insulin, probably because the mice used in this study were young and were fed a low-fat diet. Further studies of KO-supplementation using animal models of metabolic disorders and/or diets with a higher level of fat may be required to observe these effects. Frontiers Research Foundation 2011-07-12 /pmc/articles/PMC3268598/ /pubmed/22303341 http://dx.doi.org/10.3389/fgene.2011.00045 Text en Copyright © 2011 Burri, Berge, Wibrand, Berge and Barger. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
spellingShingle Genetics
Burri, Lena
Berge, Kjetil
Wibrand, Karin
Berge, Rolf K.
Barger, Jamie L.
Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title_full Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title_fullStr Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title_full_unstemmed Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title_short Differential Effects of Krill Oil and Fish Oil on the Hepatic Transcriptome in Mice
title_sort differential effects of krill oil and fish oil on the hepatic transcriptome in mice
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268598/
https://www.ncbi.nlm.nih.gov/pubmed/22303341
http://dx.doi.org/10.3389/fgene.2011.00045
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