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Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers

Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences bet...

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Autores principales: Farsani, Tayebeh Mohammadi, Etemadi, Nematollah, Sayed-Tabatabaei, Badraldin Ebrahim, Talebi, Majid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269693/
https://www.ncbi.nlm.nih.gov/pubmed/22312259
http://dx.doi.org/10.3390/ijms13010383
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author Farsani, Tayebeh Mohammadi
Etemadi, Nematollah
Sayed-Tabatabaei, Badraldin Ebrahim
Talebi, Majid
author_facet Farsani, Tayebeh Mohammadi
Etemadi, Nematollah
Sayed-Tabatabaei, Badraldin Ebrahim
Talebi, Majid
author_sort Farsani, Tayebeh Mohammadi
collection PubMed
description Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them.
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spelling pubmed-32696932012-02-06 Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers Farsani, Tayebeh Mohammadi Etemadi, Nematollah Sayed-Tabatabaei, Badraldin Ebrahim Talebi, Majid Int J Mol Sci Article Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them. Molecular Diversity Preservation International (MDPI) 2011-12-29 /pmc/articles/PMC3269693/ /pubmed/22312259 http://dx.doi.org/10.3390/ijms13010383 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Farsani, Tayebeh Mohammadi
Etemadi, Nematollah
Sayed-Tabatabaei, Badraldin Ebrahim
Talebi, Majid
Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title_full Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title_fullStr Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title_full_unstemmed Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title_short Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
title_sort assessment of genetic diversity of bermudagrass (cynodon dactylon) using issr markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269693/
https://www.ncbi.nlm.nih.gov/pubmed/22312259
http://dx.doi.org/10.3390/ijms13010383
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