Cargando…
Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers
Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences bet...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269693/ https://www.ncbi.nlm.nih.gov/pubmed/22312259 http://dx.doi.org/10.3390/ijms13010383 |
_version_ | 1782222494290149376 |
---|---|
author | Farsani, Tayebeh Mohammadi Etemadi, Nematollah Sayed-Tabatabaei, Badraldin Ebrahim Talebi, Majid |
author_facet | Farsani, Tayebeh Mohammadi Etemadi, Nematollah Sayed-Tabatabaei, Badraldin Ebrahim Talebi, Majid |
author_sort | Farsani, Tayebeh Mohammadi |
collection | PubMed |
description | Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them. |
format | Online Article Text |
id | pubmed-3269693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32696932012-02-06 Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers Farsani, Tayebeh Mohammadi Etemadi, Nematollah Sayed-Tabatabaei, Badraldin Ebrahim Talebi, Majid Int J Mol Sci Article Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them. Molecular Diversity Preservation International (MDPI) 2011-12-29 /pmc/articles/PMC3269693/ /pubmed/22312259 http://dx.doi.org/10.3390/ijms13010383 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Farsani, Tayebeh Mohammadi Etemadi, Nematollah Sayed-Tabatabaei, Badraldin Ebrahim Talebi, Majid Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title | Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title_full | Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title_fullStr | Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title_full_unstemmed | Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title_short | Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers |
title_sort | assessment of genetic diversity of bermudagrass (cynodon dactylon) using issr markers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269693/ https://www.ncbi.nlm.nih.gov/pubmed/22312259 http://dx.doi.org/10.3390/ijms13010383 |
work_keys_str_mv | AT farsanitayebehmohammadi assessmentofgeneticdiversityofbermudagrasscynodondactylonusingissrmarkers AT etemadinematollah assessmentofgeneticdiversityofbermudagrasscynodondactylonusingissrmarkers AT sayedtabatabaeibadraldinebrahim assessmentofgeneticdiversityofbermudagrasscynodondactylonusingissrmarkers AT talebimajid assessmentofgeneticdiversityofbermudagrasscynodondactylonusingissrmarkers |