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Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...

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Detalles Bibliográficos
Autores principales:	Ruslan, Rudzanna, Abd. Rahman, Raja Noor Zaliha Raja, Leow, Thean Chor, Ali, Mohd Shukuri Mohamad, Basri, Mahiran, Salleh, Abu Bakar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Molecular Diversity Preservation International (MDPI) 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269730/ https://www.ncbi.nlm.nih.gov/pubmed/22312296 http://dx.doi.org/10.3390/ijms13010943

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