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Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes)
Sulfated polysaccharides (SP) are found mainly in seaweeds and animals. To date, they have only been found in six plants and all inhabit saline environments. Furthermore, there are no reports of SP in freshwater or terrestrial plants. As such, this study investigated the presence of SP in freshwater...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269731/ https://www.ncbi.nlm.nih.gov/pubmed/22312297 http://dx.doi.org/10.3390/ijms13010961 |
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author | Dantas-Santos, Nednaldo Gomes, Dayanne Lopes Costa, Leandro Silva Cordeiro, Sara Lima Costa, Mariana Santos Santana Pereira Trindade, Edvaldo Silva Franco, Célia Regina Chavichiolo Scortecci, Kátia Castanho Leite, Edda Lisboa Rocha, Hugo Alexandre Oliveira |
author_facet | Dantas-Santos, Nednaldo Gomes, Dayanne Lopes Costa, Leandro Silva Cordeiro, Sara Lima Costa, Mariana Santos Santana Pereira Trindade, Edvaldo Silva Franco, Célia Regina Chavichiolo Scortecci, Kátia Castanho Leite, Edda Lisboa Rocha, Hugo Alexandre Oliveira |
author_sort | Dantas-Santos, Nednaldo |
collection | PubMed |
description | Sulfated polysaccharides (SP) are found mainly in seaweeds and animals. To date, they have only been found in six plants and all inhabit saline environments. Furthermore, there are no reports of SP in freshwater or terrestrial plants. As such, this study investigated the presence of SP in freshwaters Eichhornia crassipes, Egeria densa, Egeria naja, Cabomba caroliniana, Hydrocotyle bonariensis and Nymphaea ampla. Chemical analysis identified sulfate in N. ampla, H. bonariensis and, more specifically, E. crassipes. In addition, chemical analysis, FT-IR spectroscopy, histological analysis, scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDXA), as well as agarose gel electrophoresis detected SP in all parts of E. crassipes, primarily in the root (epidermis and vascular bundle). Galactose, glucose and arabinose are the main monosaccharides found in the sulfated polysaccharides from E. crassipes. In activated partial thromboplastin time (APTT) test, to evaluate the intrinsic coagulation pathway, SP from the root and rhizome prolonged the coagulation time to double the baseline value, with 0.1 mg/mL and 0.15 mg/mL, respectively. However, SP from the leaf and petiole showed no anticoagulant activity. Eichornia SP demonstrated promising anticoagulant potential and have been selected for further studies on bioguided fractionation; isolation and characterization of pure polysaccharides from this species. Additionally in vivo experiments are needed and are already underway. |
format | Online Article Text |
id | pubmed-3269731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32697312012-02-06 Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) Dantas-Santos, Nednaldo Gomes, Dayanne Lopes Costa, Leandro Silva Cordeiro, Sara Lima Costa, Mariana Santos Santana Pereira Trindade, Edvaldo Silva Franco, Célia Regina Chavichiolo Scortecci, Kátia Castanho Leite, Edda Lisboa Rocha, Hugo Alexandre Oliveira Int J Mol Sci Article Sulfated polysaccharides (SP) are found mainly in seaweeds and animals. To date, they have only been found in six plants and all inhabit saline environments. Furthermore, there are no reports of SP in freshwater or terrestrial plants. As such, this study investigated the presence of SP in freshwaters Eichhornia crassipes, Egeria densa, Egeria naja, Cabomba caroliniana, Hydrocotyle bonariensis and Nymphaea ampla. Chemical analysis identified sulfate in N. ampla, H. bonariensis and, more specifically, E. crassipes. In addition, chemical analysis, FT-IR spectroscopy, histological analysis, scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDXA), as well as agarose gel electrophoresis detected SP in all parts of E. crassipes, primarily in the root (epidermis and vascular bundle). Galactose, glucose and arabinose are the main monosaccharides found in the sulfated polysaccharides from E. crassipes. In activated partial thromboplastin time (APTT) test, to evaluate the intrinsic coagulation pathway, SP from the root and rhizome prolonged the coagulation time to double the baseline value, with 0.1 mg/mL and 0.15 mg/mL, respectively. However, SP from the leaf and petiole showed no anticoagulant activity. Eichornia SP demonstrated promising anticoagulant potential and have been selected for further studies on bioguided fractionation; isolation and characterization of pure polysaccharides from this species. Additionally in vivo experiments are needed and are already underway. Molecular Diversity Preservation International (MDPI) 2012-01-17 /pmc/articles/PMC3269731/ /pubmed/22312297 http://dx.doi.org/10.3390/ijms13010961 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Dantas-Santos, Nednaldo Gomes, Dayanne Lopes Costa, Leandro Silva Cordeiro, Sara Lima Costa, Mariana Santos Santana Pereira Trindade, Edvaldo Silva Franco, Célia Regina Chavichiolo Scortecci, Kátia Castanho Leite, Edda Lisboa Rocha, Hugo Alexandre Oliveira Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title | Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title_full | Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title_fullStr | Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title_full_unstemmed | Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title_short | Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes) |
title_sort | freshwater plants synthesize sulfated polysaccharides: heterogalactans from water hyacinth (eicchornia crassipes) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269731/ https://www.ncbi.nlm.nih.gov/pubmed/22312297 http://dx.doi.org/10.3390/ijms13010961 |
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