Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)

Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)(13)—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The...

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Autores principales: Yuan, Yanjiao, Liu, Shufang, Bai, Cuicui, Liu, Hongbo, Zhuang, Zhimeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269743/
https://www.ncbi.nlm.nih.gov/pubmed/22312309
http://dx.doi.org/10.3390/ijms13011154
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author Yuan, Yanjiao
Liu, Shufang
Bai, Cuicui
Liu, Hongbo
Zhuang, Zhimeng
author_facet Yuan, Yanjiao
Liu, Shufang
Bai, Cuicui
Liu, Hongbo
Zhuang, Zhimeng
author_sort Yuan, Yanjiao
collection PubMed
description Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)(13)—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta.
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spelling pubmed-32697432012-02-06 Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta) Yuan, Yanjiao Liu, Shufang Bai, Cuicui Liu, Hongbo Zhuang, Zhimeng Int J Mol Sci Article Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)(13)—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta. Molecular Diversity Preservation International (MDPI) 2012-01-20 /pmc/articles/PMC3269743/ /pubmed/22312309 http://dx.doi.org/10.3390/ijms13011154 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yuan, Yanjiao
Liu, Shufang
Bai, Cuicui
Liu, Hongbo
Zhuang, Zhimeng
Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title_full Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title_fullStr Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title_full_unstemmed Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title_short Isolation and Characterization of New 24 Microsatellite DNA Markers for Golden Cuttlefish (Sepia esculenta)
title_sort isolation and characterization of new 24 microsatellite dna markers for golden cuttlefish (sepia esculenta)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269743/
https://www.ncbi.nlm.nih.gov/pubmed/22312309
http://dx.doi.org/10.3390/ijms13011154
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