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microRNA Response to Listeria monocytogenes Infection in Epithelial Cells

microRNAs represent a family of very small non-coding RNAs that control several physiologic and pathologic processes, including host immune response and cancer by antagonizing a number of target mRNAs. There is limited knowledge about cell expression and the regulatory role of microRNAs following ba...

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Autores principales: Izar, Benjamin, Mannala, Gopala Krishna, Mraheil, Mobarak Abu, Chakraborty, Trinad, Hain, Torsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269745/
https://www.ncbi.nlm.nih.gov/pubmed/22312311
http://dx.doi.org/10.3390/ijms13011173
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author Izar, Benjamin
Mannala, Gopala Krishna
Mraheil, Mobarak Abu
Chakraborty, Trinad
Hain, Torsten
author_facet Izar, Benjamin
Mannala, Gopala Krishna
Mraheil, Mobarak Abu
Chakraborty, Trinad
Hain, Torsten
author_sort Izar, Benjamin
collection PubMed
description microRNAs represent a family of very small non-coding RNAs that control several physiologic and pathologic processes, including host immune response and cancer by antagonizing a number of target mRNAs. There is limited knowledge about cell expression and the regulatory role of microRNAs following bacterial infections. We investigated whether infection with a Gram-positive bacterium leads to altered expression of microRNAs involved in the host cell response in epithelial cells. Caco-2 cells were infected with Listeria monocytogenes EGD-e, a mutant strain (ΔinlAB or Δhly) or incubated with purified listeriolysin (LLO). Total RNA was isolated and microRNA and target gene expression was compared to the expression in non-infected cells using microRNA microarrays and qRT-PCR. We identified and validated five microRNAs (miR- 146b, miR-16, let-7a1, miR-145 and miR-155) that were significantly deregulated following listerial infection. We show that expression patterns of particular microRNAs strongly depend on pathogen localization and the presence of bacterial effector proteins. Strikingly, miR-155 which was shown to have an important role in inflammatory responses during infection was induced by wild-type bacteria, by LLO-deficient bacteria and following incubation with purified LLO. It was downregulated following ΔinlAB infection indicating a new potent role for internalins in listerial pathogenicity and miRNA regulation. Concurrently, we observed differences in target transcript expression of the investigated miRNAs. We provide first evidence that L. monocytogenes infection leads to deregulation of a set of microRNAs with important roles in host response. Distinct microRNA expression depends on both LLO and pathogen localization.
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spelling pubmed-32697452012-02-06 microRNA Response to Listeria monocytogenes Infection in Epithelial Cells Izar, Benjamin Mannala, Gopala Krishna Mraheil, Mobarak Abu Chakraborty, Trinad Hain, Torsten Int J Mol Sci Article microRNAs represent a family of very small non-coding RNAs that control several physiologic and pathologic processes, including host immune response and cancer by antagonizing a number of target mRNAs. There is limited knowledge about cell expression and the regulatory role of microRNAs following bacterial infections. We investigated whether infection with a Gram-positive bacterium leads to altered expression of microRNAs involved in the host cell response in epithelial cells. Caco-2 cells were infected with Listeria monocytogenes EGD-e, a mutant strain (ΔinlAB or Δhly) or incubated with purified listeriolysin (LLO). Total RNA was isolated and microRNA and target gene expression was compared to the expression in non-infected cells using microRNA microarrays and qRT-PCR. We identified and validated five microRNAs (miR- 146b, miR-16, let-7a1, miR-145 and miR-155) that were significantly deregulated following listerial infection. We show that expression patterns of particular microRNAs strongly depend on pathogen localization and the presence of bacterial effector proteins. Strikingly, miR-155 which was shown to have an important role in inflammatory responses during infection was induced by wild-type bacteria, by LLO-deficient bacteria and following incubation with purified LLO. It was downregulated following ΔinlAB infection indicating a new potent role for internalins in listerial pathogenicity and miRNA regulation. Concurrently, we observed differences in target transcript expression of the investigated miRNAs. We provide first evidence that L. monocytogenes infection leads to deregulation of a set of microRNAs with important roles in host response. Distinct microRNA expression depends on both LLO and pathogen localization. Molecular Diversity Preservation International (MDPI) 2012-01-20 /pmc/articles/PMC3269745/ /pubmed/22312311 http://dx.doi.org/10.3390/ijms13011173 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Izar, Benjamin
Mannala, Gopala Krishna
Mraheil, Mobarak Abu
Chakraborty, Trinad
Hain, Torsten
microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title_full microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title_fullStr microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title_full_unstemmed microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title_short microRNA Response to Listeria monocytogenes Infection in Epithelial Cells
title_sort microrna response to listeria monocytogenes infection in epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269745/
https://www.ncbi.nlm.nih.gov/pubmed/22312311
http://dx.doi.org/10.3390/ijms13011173
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AT chakrabortytrinad micrornaresponsetolisteriamonocytogenesinfectioninepithelialcells
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