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Correlating light scattering with internal cellular structures

The origins of side scattering from a fibroblast and cervical cell line were determined by comparing side-scatter images with images stained for lysosomes, nuclei, and mitochondria on a cell by cell basis. Lysosomes or nuclei are the most efficient type of scatterer depending on the cell type and in...

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Autores principales: Marina, Oana C., Sanders, Claire K., Mourant, Judith R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269847/
https://www.ncbi.nlm.nih.gov/pubmed/22312583
http://dx.doi.org/10.1364/BOE.3.000296
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author Marina, Oana C.
Sanders, Claire K.
Mourant, Judith R.
author_facet Marina, Oana C.
Sanders, Claire K.
Mourant, Judith R.
author_sort Marina, Oana C.
collection PubMed
description The origins of side scattering from a fibroblast and cervical cell line were determined by comparing side-scatter images with images stained for lysosomes, nuclei, and mitochondria on a cell by cell basis. Lysosomes or nuclei are the most efficient type of scatterer depending on the cell type and incident light polarization. The relative scattering efficiencies of lysosomes and mitochondria were the same for both cell lines, while the scattering efficiencies of the nuclei differed. The percent of 90° scattering from the nucleus, mitochondria, and lysosomes as well as the group of other internal cellular objects was estimated. The nucleus was the largest contributor to side scatter in the cervical carcinoma cells. The contributions of lysosomes, mitochondria, the nucleus, and particles unstained by either Hoechst, LysoSensor or MitoTracker ranges from ∼20% to ∼30% in fibroblast cells. The contribution of lysosomes to side scatter was much stronger when the incident light was polarized perpendicular to the scattering plane than when the polarization of the side scatter laser was parallel to the scattering plane. This dependence on side scatter polarization indicates that lysosomes contain scattering structures that are much smaller than the wavelength of light used in the measurements (785 nm). In conclusion, mitochondria were not found to be either the most efficient scatterer or to have the largest contribution to scattering in either cell line, in contrast to previous reports. Rather lysosomes, nuclei and unknown particles all have significant contributions to 90° scattering and the contributions of some of these particles can be modulated by changing the polarization of the incident light.
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spelling pubmed-32698472012-02-06 Correlating light scattering with internal cellular structures Marina, Oana C. Sanders, Claire K. Mourant, Judith R. Biomed Opt Express Cell Studies The origins of side scattering from a fibroblast and cervical cell line were determined by comparing side-scatter images with images stained for lysosomes, nuclei, and mitochondria on a cell by cell basis. Lysosomes or nuclei are the most efficient type of scatterer depending on the cell type and incident light polarization. The relative scattering efficiencies of lysosomes and mitochondria were the same for both cell lines, while the scattering efficiencies of the nuclei differed. The percent of 90° scattering from the nucleus, mitochondria, and lysosomes as well as the group of other internal cellular objects was estimated. The nucleus was the largest contributor to side scatter in the cervical carcinoma cells. The contributions of lysosomes, mitochondria, the nucleus, and particles unstained by either Hoechst, LysoSensor or MitoTracker ranges from ∼20% to ∼30% in fibroblast cells. The contribution of lysosomes to side scatter was much stronger when the incident light was polarized perpendicular to the scattering plane than when the polarization of the side scatter laser was parallel to the scattering plane. This dependence on side scatter polarization indicates that lysosomes contain scattering structures that are much smaller than the wavelength of light used in the measurements (785 nm). In conclusion, mitochondria were not found to be either the most efficient scatterer or to have the largest contribution to scattering in either cell line, in contrast to previous reports. Rather lysosomes, nuclei and unknown particles all have significant contributions to 90° scattering and the contributions of some of these particles can be modulated by changing the polarization of the incident light. Optical Society of America 2012-01-13 /pmc/articles/PMC3269847/ /pubmed/22312583 http://dx.doi.org/10.1364/BOE.3.000296 Text en © 2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Cell Studies
Marina, Oana C.
Sanders, Claire K.
Mourant, Judith R.
Correlating light scattering with internal cellular structures
title Correlating light scattering with internal cellular structures
title_full Correlating light scattering with internal cellular structures
title_fullStr Correlating light scattering with internal cellular structures
title_full_unstemmed Correlating light scattering with internal cellular structures
title_short Correlating light scattering with internal cellular structures
title_sort correlating light scattering with internal cellular structures
topic Cell Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269847/
https://www.ncbi.nlm.nih.gov/pubmed/22312583
http://dx.doi.org/10.1364/BOE.3.000296
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