Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can b...

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Autores principales: Watanabe, Koyo, Matsuura, Koji, Kawata, Fukukazu, Nagata, Kotaro, Ning, Jun, Kano, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2012
Materias:
Cell Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269851/
https://www.ncbi.nlm.nih.gov/pubmed/22312587
http://dx.doi.org/10.1364/BOE.3.000354
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author Watanabe, Koyo
Matsuura, Koji
Kawata, Fukukazu
Nagata, Kotaro
Ning, Jun
Kano, Hiroshi
author_facet Watanabe, Koyo
Matsuura, Koji
Kawata, Fukukazu
Nagata, Kotaro
Ning, Jun
Kano, Hiroshi
author_sort Watanabe, Koyo
collection PubMed
description We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.
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spelling pubmed-32698512012-02-06 Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites Watanabe, Koyo Matsuura, Koji Kawata, Fukukazu Nagata, Kotaro Ning, Jun Kano, Hiroshi Biomed Opt Express Cell Studies We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest. Optical Society of America 2012-01-23 /pmc/articles/PMC3269851/ /pubmed/22312587 http://dx.doi.org/10.1364/BOE.3.000354 Text en ©2012 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Cell Studies
Watanabe, Koyo
Matsuura, Koji
Kawata, Fukukazu
Nagata, Kotaro
Ning, Jun
Kano, Hiroshi
Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title_full Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title_fullStr Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title_full_unstemmed Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title_short Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
title_sort scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
topic Cell Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269851/
https://www.ncbi.nlm.nih.gov/pubmed/22312587
http://dx.doi.org/10.1364/BOE.3.000354
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