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Endothelial and perivascular cells maintain haematopoietic stem cells
Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270376/ https://www.ncbi.nlm.nih.gov/pubmed/22281595 http://dx.doi.org/10.1038/nature10783 |
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author | Ding, Lei Saunders, Thomas L. Enikolopov, Grigori Morrison, Sean J. |
author_facet | Ding, Lei Saunders, Thomas L. Enikolopov, Grigori Morrison, Sean J. |
author_sort | Ding, Lei |
collection | PubMed |
description | Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem Cell Factor (SCF) is a key niche component that maintains HSCs. Using Scf(gfp) knock-in mice we found Scf was primarily expressed by perivascular cells throughout bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, Nestin-Cre, or Nestin-CreER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or Leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance. |
format | Online Article Text |
id | pubmed-3270376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-32703762012-07-26 Endothelial and perivascular cells maintain haematopoietic stem cells Ding, Lei Saunders, Thomas L. Enikolopov, Grigori Morrison, Sean J. Nature Article Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem Cell Factor (SCF) is a key niche component that maintains HSCs. Using Scf(gfp) knock-in mice we found Scf was primarily expressed by perivascular cells throughout bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, Nestin-Cre, or Nestin-CreER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or Leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance. 2012-01-25 /pmc/articles/PMC3270376/ /pubmed/22281595 http://dx.doi.org/10.1038/nature10783 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Ding, Lei Saunders, Thomas L. Enikolopov, Grigori Morrison, Sean J. Endothelial and perivascular cells maintain haematopoietic stem cells |
title | Endothelial and perivascular cells maintain haematopoietic stem cells |
title_full | Endothelial and perivascular cells maintain haematopoietic stem cells |
title_fullStr | Endothelial and perivascular cells maintain haematopoietic stem cells |
title_full_unstemmed | Endothelial and perivascular cells maintain haematopoietic stem cells |
title_short | Endothelial and perivascular cells maintain haematopoietic stem cells |
title_sort | endothelial and perivascular cells maintain haematopoietic stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270376/ https://www.ncbi.nlm.nih.gov/pubmed/22281595 http://dx.doi.org/10.1038/nature10783 |
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