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Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway

Nanostructured, self-assembling peptides hold promise for a variety of regenerative medical applications such as 3D cell culture systems, accelerated wound healing, and nerve repair. The aim of this study was to determine whether the self-assembling peptide K5 can be applied as a carrier of anti-inf...

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Autores principales: Yang, Woo Seok, Park, Yung Chul, Kim, Ji Hye, Kim, Hye Ri, Yu, Tao, Byeon, Se Eun, Unsworth, Larry D., Lee, Jaehwi, Cho, Jae Youl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270444/
https://www.ncbi.nlm.nih.gov/pubmed/22315508
http://dx.doi.org/10.1155/2012/489810
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author Yang, Woo Seok
Park, Yung Chul
Kim, Ji Hye
Kim, Hye Ri
Yu, Tao
Byeon, Se Eun
Unsworth, Larry D.
Lee, Jaehwi
Cho, Jae Youl
author_facet Yang, Woo Seok
Park, Yung Chul
Kim, Ji Hye
Kim, Hye Ri
Yu, Tao
Byeon, Se Eun
Unsworth, Larry D.
Lee, Jaehwi
Cho, Jae Youl
author_sort Yang, Woo Seok
collection PubMed
description Nanostructured, self-assembling peptides hold promise for a variety of regenerative medical applications such as 3D cell culture systems, accelerated wound healing, and nerve repair. The aim of this study was to determine whether the self-assembling peptide K5 can be applied as a carrier of anti-inflammatory drugs. First, we examined whether the K5 self-assembling peptide itself can modulate various cellular inflammatory responses. We found that peptide K5 significantly suppressed the release of tumor-necrosis-factor- (TNF-) α and prostaglandin E(2) (PGE(2)) from RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS). Similarly, there was inhibition of cyclooxygenase- (COX-) 2 mRNA expression assessed by real-time PCR, indicating that the inhibition is at the transcriptional level. In agreement with this finding, peptide K5 suppressed the translocation of the transcription factors activator protein (AP-1) and c-Jun and inhibited upstream inflammatory effectors including mitogen activated protein kinase (MAPK), p38, and mitogen-activated protein kinase kinase 3/6 (MKK 3/6). Whether this peptide exerts its effects via a transmembrane or cytoplasmic receptor is not clear. However, our data strongly suggest that the nanostructured, self-assembling peptide K5 may possess significant anti-inflammatory activity via suppression of the p38/AP-1 pathway.
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spelling pubmed-32704442012-02-07 Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway Yang, Woo Seok Park, Yung Chul Kim, Ji Hye Kim, Hye Ri Yu, Tao Byeon, Se Eun Unsworth, Larry D. Lee, Jaehwi Cho, Jae Youl Mediators Inflamm Research Article Nanostructured, self-assembling peptides hold promise for a variety of regenerative medical applications such as 3D cell culture systems, accelerated wound healing, and nerve repair. The aim of this study was to determine whether the self-assembling peptide K5 can be applied as a carrier of anti-inflammatory drugs. First, we examined whether the K5 self-assembling peptide itself can modulate various cellular inflammatory responses. We found that peptide K5 significantly suppressed the release of tumor-necrosis-factor- (TNF-) α and prostaglandin E(2) (PGE(2)) from RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS). Similarly, there was inhibition of cyclooxygenase- (COX-) 2 mRNA expression assessed by real-time PCR, indicating that the inhibition is at the transcriptional level. In agreement with this finding, peptide K5 suppressed the translocation of the transcription factors activator protein (AP-1) and c-Jun and inhibited upstream inflammatory effectors including mitogen activated protein kinase (MAPK), p38, and mitogen-activated protein kinase kinase 3/6 (MKK 3/6). Whether this peptide exerts its effects via a transmembrane or cytoplasmic receptor is not clear. However, our data strongly suggest that the nanostructured, self-assembling peptide K5 may possess significant anti-inflammatory activity via suppression of the p38/AP-1 pathway. Hindawi Publishing Corporation 2012 2012-01-24 /pmc/articles/PMC3270444/ /pubmed/22315508 http://dx.doi.org/10.1155/2012/489810 Text en Copyright © 2012 Woo Seok Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Woo Seok
Park, Yung Chul
Kim, Ji Hye
Kim, Hye Ri
Yu, Tao
Byeon, Se Eun
Unsworth, Larry D.
Lee, Jaehwi
Cho, Jae Youl
Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title_full Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title_fullStr Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title_full_unstemmed Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title_short Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE(2) Production by Suppression of the AP-1/p38 Pathway
title_sort nanostructured, self-assembling peptide k5 blocks tnf-α and pge(2) production by suppression of the ap-1/p38 pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270444/
https://www.ncbi.nlm.nih.gov/pubmed/22315508
http://dx.doi.org/10.1155/2012/489810
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