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1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem

The aim of the present study was to assess aquatic contamination by polycyclic aromatic hydrocarbons (PAH), using the 1-hydroxypyrene (1-OHP) content in fish bile as a biochemical marker. A total of 71 chub (Leuciscus cephalus L.) were collected from seven locations on the Svitava and Svratka rivers...

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Autores principales: Blahova, Jana, Kruzikova, Kamila, Kasiková, Barbora, Stierand, Pavel, Jurcikova, Jana, Ocelka, Tomas, Svobodova, Zdenka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270836/
https://www.ncbi.nlm.nih.gov/pubmed/22315535
http://dx.doi.org/10.3390/s100100203
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author Blahova, Jana
Kruzikova, Kamila
Kasiková, Barbora
Stierand, Pavel
Jurcikova, Jana
Ocelka, Tomas
Svobodova, Zdenka
author_facet Blahova, Jana
Kruzikova, Kamila
Kasiková, Barbora
Stierand, Pavel
Jurcikova, Jana
Ocelka, Tomas
Svobodova, Zdenka
author_sort Blahova, Jana
collection PubMed
description The aim of the present study was to assess aquatic contamination by polycyclic aromatic hydrocarbons (PAH), using the 1-hydroxypyrene (1-OHP) content in fish bile as a biochemical marker. A total of 71 chub (Leuciscus cephalus L.) were collected from seven locations on the Svitava and Svratka rivers in and around the industrial city of Brno, Czech Republic. The levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection after deconjugation. Normalising the molar concentration of the biliary 1-OHP to the biliary protein content reduced sample variation. The content of 1-OHP was correlated with the PAH level in bottom sediment and semi-permeable membrane devices (SPMD), which was analyzed by a combination of HPLC/FLD and GC/MS methods. The highest mean values of 1-OHP were found in fish caught at the Svratka River at locations Modřice (169.2 ± 99.7 ng·mg(−1) protein) and Rajhradice (152.2 ± 79.7 ng·mg(−1) protein), which are located downstream from Brno. These values were significantly (P < 0.05) higher than those obtained from localities Kníničky (98.4 ± 66.1 ng·mg(−1) protein) and Bílovice nad Svitavou (64.1 ± 31.4 ng·mg(−1) protein). The lowest contents of PAH in sediment and SPMD were found at location Kníničky (1.5 mg·kg(−1) dry mass and 19.4 ng·L(−1), respectively). The highest contents of PAH in sediment and SPMD were found in Rajhradice (26.0 mg·kg(−1) dry mass) and Svitava before junction (65.4 ng·L(−1)), respectively. A Spearman correlation test was applied to determine the relationship between biliary 1-OHP and the sum of PAH in sediment and SPMD. A positive, but no statistically significant correlation was found. The main impact sources of elevated level of PAHs in sites located downstream from Brno are most probably intensive industrial and agricultural activities and domestic waste.
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spelling pubmed-32708362012-02-07 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem Blahova, Jana Kruzikova, Kamila Kasiková, Barbora Stierand, Pavel Jurcikova, Jana Ocelka, Tomas Svobodova, Zdenka Sensors (Basel) Article The aim of the present study was to assess aquatic contamination by polycyclic aromatic hydrocarbons (PAH), using the 1-hydroxypyrene (1-OHP) content in fish bile as a biochemical marker. A total of 71 chub (Leuciscus cephalus L.) were collected from seven locations on the Svitava and Svratka rivers in and around the industrial city of Brno, Czech Republic. The levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection after deconjugation. Normalising the molar concentration of the biliary 1-OHP to the biliary protein content reduced sample variation. The content of 1-OHP was correlated with the PAH level in bottom sediment and semi-permeable membrane devices (SPMD), which was analyzed by a combination of HPLC/FLD and GC/MS methods. The highest mean values of 1-OHP were found in fish caught at the Svratka River at locations Modřice (169.2 ± 99.7 ng·mg(−1) protein) and Rajhradice (152.2 ± 79.7 ng·mg(−1) protein), which are located downstream from Brno. These values were significantly (P < 0.05) higher than those obtained from localities Kníničky (98.4 ± 66.1 ng·mg(−1) protein) and Bílovice nad Svitavou (64.1 ± 31.4 ng·mg(−1) protein). The lowest contents of PAH in sediment and SPMD were found at location Kníničky (1.5 mg·kg(−1) dry mass and 19.4 ng·L(−1), respectively). The highest contents of PAH in sediment and SPMD were found in Rajhradice (26.0 mg·kg(−1) dry mass) and Svitava before junction (65.4 ng·L(−1)), respectively. A Spearman correlation test was applied to determine the relationship between biliary 1-OHP and the sum of PAH in sediment and SPMD. A positive, but no statistically significant correlation was found. The main impact sources of elevated level of PAHs in sites located downstream from Brno are most probably intensive industrial and agricultural activities and domestic waste. Molecular Diversity Preservation International (MDPI) 2009-12-28 /pmc/articles/PMC3270836/ /pubmed/22315535 http://dx.doi.org/10.3390/s100100203 Text en ©2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
spellingShingle Article
Blahova, Jana
Kruzikova, Kamila
Kasiková, Barbora
Stierand, Pavel
Jurcikova, Jana
Ocelka, Tomas
Svobodova, Zdenka
1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title_full 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title_fullStr 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title_full_unstemmed 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title_short 1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic Ecosystem
title_sort 1-hydroxypyrene–a biochemical marker for pah pollution assessment of aquatic ecosystem
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3270836/
https://www.ncbi.nlm.nih.gov/pubmed/22315535
http://dx.doi.org/10.3390/s100100203
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