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Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment

In this study, both culture-dependent and culture-independent methods were used to determine whether the iron sulfide mineral- and nitrate-rich freshwater nature reserve Het Zwart Water accommodates anoxic microbial iron cycling. Molecular analyses (16S rRNA gene clone library and fluorescence in si...

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Autores principales: Haaijer, Suzanne C. M., Crienen, Gijs, Jetten, Mike S. M., Op den Camp, Huub J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271277/
https://www.ncbi.nlm.nih.gov/pubmed/22347219
http://dx.doi.org/10.3389/fmicb.2012.00026
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author Haaijer, Suzanne C. M.
Crienen, Gijs
Jetten, Mike S. M.
Op den Camp, Huub J. M.
author_facet Haaijer, Suzanne C. M.
Crienen, Gijs
Jetten, Mike S. M.
Op den Camp, Huub J. M.
author_sort Haaijer, Suzanne C. M.
collection PubMed
description In this study, both culture-dependent and culture-independent methods were used to determine whether the iron sulfide mineral- and nitrate-rich freshwater nature reserve Het Zwart Water accommodates anoxic microbial iron cycling. Molecular analyses (16S rRNA gene clone library and fluorescence in situ hybridization, FISH) showed that sulfur-oxidizing denitrifiers dominated the microbial population. In addition, bacteria resembling the iron-oxidizing, nitrate-reducing Acidovorax strain BrG1 accounted for a major part of the microbial community in the groundwater of this ecosystem. Despite the apparent abundance of strain BrG1-like bacteria, iron-oxidizing nitrate reducers could not be isolated, likely due to the strictly autotrophic cultivation conditions adopted in our study. In contrast an iron-reducing Geobacter sp. was isolated from this environment while FISH and 16S rRNA gene clone library analyses did not reveal any Geobacter sp.-related sequences in the groundwater. Our findings indicate that iron-oxidizing nitrate reducers may be of importance to the redox cycling of iron in the groundwater of our study site and illustrate the necessity of employing both culture-dependent and independent methods in studies on microbial processes.
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spelling pubmed-32712772012-02-15 Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment Haaijer, Suzanne C. M. Crienen, Gijs Jetten, Mike S. M. Op den Camp, Huub J. M. Front Microbiol Microbiology In this study, both culture-dependent and culture-independent methods were used to determine whether the iron sulfide mineral- and nitrate-rich freshwater nature reserve Het Zwart Water accommodates anoxic microbial iron cycling. Molecular analyses (16S rRNA gene clone library and fluorescence in situ hybridization, FISH) showed that sulfur-oxidizing denitrifiers dominated the microbial population. In addition, bacteria resembling the iron-oxidizing, nitrate-reducing Acidovorax strain BrG1 accounted for a major part of the microbial community in the groundwater of this ecosystem. Despite the apparent abundance of strain BrG1-like bacteria, iron-oxidizing nitrate reducers could not be isolated, likely due to the strictly autotrophic cultivation conditions adopted in our study. In contrast an iron-reducing Geobacter sp. was isolated from this environment while FISH and 16S rRNA gene clone library analyses did not reveal any Geobacter sp.-related sequences in the groundwater. Our findings indicate that iron-oxidizing nitrate reducers may be of importance to the redox cycling of iron in the groundwater of our study site and illustrate the necessity of employing both culture-dependent and independent methods in studies on microbial processes. Frontiers Research Foundation 2012-02-03 /pmc/articles/PMC3271277/ /pubmed/22347219 http://dx.doi.org/10.3389/fmicb.2012.00026 Text en Copyright © 2012 Haaijer, Crienen, Jetten and Op den Camp. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
spellingShingle Microbiology
Haaijer, Suzanne C. M.
Crienen, Gijs
Jetten, Mike S. M.
Op den Camp, Huub J. M.
Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title_full Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title_fullStr Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title_full_unstemmed Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title_short Anoxic Iron Cycling Bacteria from an Iron Sulfide- and Nitrate-Rich Freshwater Environment
title_sort anoxic iron cycling bacteria from an iron sulfide- and nitrate-rich freshwater environment
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271277/
https://www.ncbi.nlm.nih.gov/pubmed/22347219
http://dx.doi.org/10.3389/fmicb.2012.00026
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