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Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification
CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneou...
Autores principales: | Xu, Gaolian, Hu, Lin, Zhong, Huayan, Wang, Hongying, Yusa, Sei-ichi, Weiss, Tristen C., Romaniuk, Paul J., Pickerill, Sam, You, Qimin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271364/ https://www.ncbi.nlm.nih.gov/pubmed/22355758 http://dx.doi.org/10.1038/srep00246 |
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