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Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies

Next-generation sequencing of transcriptomes (RNA-Seq) is being used increasingly in studies of nonmodel organisms. Here, we evaluate the effectiveness of normalising cDNA libraries prior to sequencing in a small-scale study of the zebra finch. We find that assemblies produced from normalised librar...

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Detalles Bibliográficos
Autores principales: Ekblom, Robert, Slate, Jon, Horsburgh, Gavin J., Birkhead, Tim, Burke, Terry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272792/
https://www.ncbi.nlm.nih.gov/pubmed/22319409
http://dx.doi.org/10.1155/2012/281693