Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts

Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). I...

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Autores principales: Prosecká, Eva, Buzgo, Matej, Rampichová, Michala, Kocourek, Tomáš, Kochová, Petra, Vysloužilová, Lucie, Tvrdík, Daniel, Jelínek, Miroslav, Lukáš, David, Amler, Evžen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272836/
https://www.ncbi.nlm.nih.gov/pubmed/22319242
http://dx.doi.org/10.1155/2012/428503
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author Prosecká, Eva
Buzgo, Matej
Rampichová, Michala
Kocourek, Tomáš
Kochová, Petra
Vysloužilová, Lucie
Tvrdík, Daniel
Jelínek, Miroslav
Lukáš, David
Amler, Evžen
author_facet Prosecká, Eva
Buzgo, Matej
Rampichová, Michala
Kocourek, Tomáš
Kochová, Petra
Vysloužilová, Lucie
Tvrdík, Daniel
Jelínek, Miroslav
Lukáš, David
Amler, Evžen
author_sort Prosecká, Eva
collection PubMed
description Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
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spelling pubmed-32728362012-02-08 Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts Prosecká, Eva Buzgo, Matej Rampichová, Michala Kocourek, Tomáš Kochová, Petra Vysloužilová, Lucie Tvrdík, Daniel Jelínek, Miroslav Lukáš, David Amler, Evžen J Biomed Biotechnol Research Article Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm. Hindawi Publishing Corporation 2012 2012-01-29 /pmc/articles/PMC3272836/ /pubmed/22319242 http://dx.doi.org/10.1155/2012/428503 Text en Copyright © 2012 Eva Prosecká et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Prosecká, Eva
Buzgo, Matej
Rampichová, Michala
Kocourek, Tomáš
Kochová, Petra
Vysloužilová, Lucie
Tvrdík, Daniel
Jelínek, Miroslav
Lukáš, David
Amler, Evžen
Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title_full Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title_fullStr Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title_full_unstemmed Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title_short Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
title_sort thin-layer hydroxyapatite deposition on a nanofiber surface stimulates mesenchymal stem cell proliferation and their differentiation into osteoblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272836/
https://www.ncbi.nlm.nih.gov/pubmed/22319242
http://dx.doi.org/10.1155/2012/428503
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