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NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression

Pluripotent cells possess the ability to differentiate into any cell type. Commitment to differentiate into specific lineages requires strict control of gene expression to coordinate the downregulation of lineage inappropriate genes while enabling the expression of lineage-specific genes. The nucleo...

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Autores principales: Reynolds, Nicola, Salmon-Divon, Mali, Dvinge, Heidi, Hynes-Allen, Antony, Balasooriya, Gayan, Leaford, Donna, Behrens, Axel, Bertone, Paul, Hendrich, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273378/
https://www.ncbi.nlm.nih.gov/pubmed/22139358
http://dx.doi.org/10.1038/emboj.2011.431
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author Reynolds, Nicola
Salmon-Divon, Mali
Dvinge, Heidi
Hynes-Allen, Antony
Balasooriya, Gayan
Leaford, Donna
Behrens, Axel
Bertone, Paul
Hendrich, Brian
author_facet Reynolds, Nicola
Salmon-Divon, Mali
Dvinge, Heidi
Hynes-Allen, Antony
Balasooriya, Gayan
Leaford, Donna
Behrens, Axel
Bertone, Paul
Hendrich, Brian
author_sort Reynolds, Nicola
collection PubMed
description Pluripotent cells possess the ability to differentiate into any cell type. Commitment to differentiate into specific lineages requires strict control of gene expression to coordinate the downregulation of lineage inappropriate genes while enabling the expression of lineage-specific genes. The nucleosome remodelling and deacetylation complex (NuRD) is required for lineage commitment of pluripotent cells; however, the mechanism through which it exerts this effect has not been defined. Here, we show that histone deacetylation by NuRD specifies recruitment for Polycomb Repressive Complex 2 (PRC2) in embryonic stem (ES) cells. NuRD-mediated deacetylation of histone H3K27 enables PRC2 recruitment and subsequent H3K27 trimethylation at NuRD target promoters. We propose a gene-specific mechanism for modulating expression of transcriptionally poised genes whereby NuRD controls the balance between acetylation and methylation of histones, thereby precisely directing the expression of genes critical for embryonic development.
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spelling pubmed-32733782012-02-06 NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression Reynolds, Nicola Salmon-Divon, Mali Dvinge, Heidi Hynes-Allen, Antony Balasooriya, Gayan Leaford, Donna Behrens, Axel Bertone, Paul Hendrich, Brian EMBO J Article Pluripotent cells possess the ability to differentiate into any cell type. Commitment to differentiate into specific lineages requires strict control of gene expression to coordinate the downregulation of lineage inappropriate genes while enabling the expression of lineage-specific genes. The nucleosome remodelling and deacetylation complex (NuRD) is required for lineage commitment of pluripotent cells; however, the mechanism through which it exerts this effect has not been defined. Here, we show that histone deacetylation by NuRD specifies recruitment for Polycomb Repressive Complex 2 (PRC2) in embryonic stem (ES) cells. NuRD-mediated deacetylation of histone H3K27 enables PRC2 recruitment and subsequent H3K27 trimethylation at NuRD target promoters. We propose a gene-specific mechanism for modulating expression of transcriptionally poised genes whereby NuRD controls the balance between acetylation and methylation of histones, thereby precisely directing the expression of genes critical for embryonic development. European Molecular Biology Organization 2012-02-01 2011-12-02 /pmc/articles/PMC3273378/ /pubmed/22139358 http://dx.doi.org/10.1038/emboj.2011.431 Text en Copyright © 2012, European Molecular Biology Organization https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Article
Reynolds, Nicola
Salmon-Divon, Mali
Dvinge, Heidi
Hynes-Allen, Antony
Balasooriya, Gayan
Leaford, Donna
Behrens, Axel
Bertone, Paul
Hendrich, Brian
NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title_full NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title_fullStr NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title_full_unstemmed NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title_short NuRD-mediated deacetylation of H3K27 facilitates recruitment of Polycomb Repressive Complex 2 to direct gene repression
title_sort nurd-mediated deacetylation of h3k27 facilitates recruitment of polycomb repressive complex 2 to direct gene repression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273378/
https://www.ncbi.nlm.nih.gov/pubmed/22139358
http://dx.doi.org/10.1038/emboj.2011.431
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