Cargando…

Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10

The 2009 influenza pandemic provided an opportunity to observe dynamic changes of the hemagglutinin (HA) and neuraminidase (NA) of pH1N1 strains that spread in two metropolitan areas -Taipei and Kaohsiung. We observed cumulative increases of amino acid substitutions of both HA and NA that were highe...

Descripción completa

Detalles Bibliográficos
Autores principales: Kao, Chuan-Liang, Chan, Ta-Chien, Tsai, Chu-Han, Chu, Kuan-Ying, Chuang, Shu-Fang, Lee, Chang-Chun, Li, Zheng-Rong Tiger, Wu, Ko-Wen, Chang, Luan-Yin, Shen, Yea-Huei, Huang, Li-Min, Lee, Ping-Ing, Yang, ChingLai, Compans, Richard, Rouse, Barry T., King, Chwan-Chuen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273476/
https://www.ncbi.nlm.nih.gov/pubmed/22328930
http://dx.doi.org/10.1371/journal.pone.0031162
_version_ 1782222945004814336
author Kao, Chuan-Liang
Chan, Ta-Chien
Tsai, Chu-Han
Chu, Kuan-Ying
Chuang, Shu-Fang
Lee, Chang-Chun
Li, Zheng-Rong Tiger
Wu, Ko-Wen
Chang, Luan-Yin
Shen, Yea-Huei
Huang, Li-Min
Lee, Ping-Ing
Yang, ChingLai
Compans, Richard
Rouse, Barry T.
King, Chwan-Chuen
author_facet Kao, Chuan-Liang
Chan, Ta-Chien
Tsai, Chu-Han
Chu, Kuan-Ying
Chuang, Shu-Fang
Lee, Chang-Chun
Li, Zheng-Rong Tiger
Wu, Ko-Wen
Chang, Luan-Yin
Shen, Yea-Huei
Huang, Li-Min
Lee, Ping-Ing
Yang, ChingLai
Compans, Richard
Rouse, Barry T.
King, Chwan-Chuen
author_sort Kao, Chuan-Liang
collection PubMed
description The 2009 influenza pandemic provided an opportunity to observe dynamic changes of the hemagglutinin (HA) and neuraminidase (NA) of pH1N1 strains that spread in two metropolitan areas -Taipei and Kaohsiung. We observed cumulative increases of amino acid substitutions of both HA and NA that were higher in the post–peak than in the pre-peak period of the epidemic. About 14.94% and 3.44% of 174 isolates had one and two amino acids changes, respective, in the four antigenic sites. One unique adaptive mutation of HA2 (E374K) was first detected three weeks before the epidemic peak. This mutation evolved through the epidemic, and finally emerged as the major circulated strain, with significantly higher frequency in the post-peak period than in the pre-peak (64.65% vs 9.28%, p<0.0001). E374K persisted until ten months post-nationwide vaccination without further antigenic changes (e.g. prior to the highest selective pressure). In public health measures, the epidemic peaked at seven weeks after oseltamivir treatment was initiated. The emerging E374K mutants spread before the first peak of school class suspension, extended their survival in high-density population areas before vaccination, dominated in the second wave of class suspension, and were fixed as herd immunity developed. The tempo-spatial spreading of E374K mutants was more concentrated during the post–peak (p = 0.000004) in seven districts with higher spatial clusters (p<0.001). This is the first study examining viral changes during the naïve phase of a pandemic of influenza through integrated virological/serological/clinical surveillance, tempo-spatial analysis, and intervention policies. The vaccination increased the percentage of E374K mutants (22.86% vs 72.34%, p<0.001) and significantly elevated the frequency of mutations in Sa antigenic site (2.36% vs 23.40%, p<0.001). Future pre-vaccination public health efforts should monitor amino acids of HA and NA of pandemic influenza viruses isolated at exponential and peak phases in areas with high cluster cases.
format Online
Article
Text
id pubmed-3273476
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-32734762012-02-10 Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10 Kao, Chuan-Liang Chan, Ta-Chien Tsai, Chu-Han Chu, Kuan-Ying Chuang, Shu-Fang Lee, Chang-Chun Li, Zheng-Rong Tiger Wu, Ko-Wen Chang, Luan-Yin Shen, Yea-Huei Huang, Li-Min Lee, Ping-Ing Yang, ChingLai Compans, Richard Rouse, Barry T. King, Chwan-Chuen PLoS One Research Article The 2009 influenza pandemic provided an opportunity to observe dynamic changes of the hemagglutinin (HA) and neuraminidase (NA) of pH1N1 strains that spread in two metropolitan areas -Taipei and Kaohsiung. We observed cumulative increases of amino acid substitutions of both HA and NA that were higher in the post–peak than in the pre-peak period of the epidemic. About 14.94% and 3.44% of 174 isolates had one and two amino acids changes, respective, in the four antigenic sites. One unique adaptive mutation of HA2 (E374K) was first detected three weeks before the epidemic peak. This mutation evolved through the epidemic, and finally emerged as the major circulated strain, with significantly higher frequency in the post-peak period than in the pre-peak (64.65% vs 9.28%, p<0.0001). E374K persisted until ten months post-nationwide vaccination without further antigenic changes (e.g. prior to the highest selective pressure). In public health measures, the epidemic peaked at seven weeks after oseltamivir treatment was initiated. The emerging E374K mutants spread before the first peak of school class suspension, extended their survival in high-density population areas before vaccination, dominated in the second wave of class suspension, and were fixed as herd immunity developed. The tempo-spatial spreading of E374K mutants was more concentrated during the post–peak (p = 0.000004) in seven districts with higher spatial clusters (p<0.001). This is the first study examining viral changes during the naïve phase of a pandemic of influenza through integrated virological/serological/clinical surveillance, tempo-spatial analysis, and intervention policies. The vaccination increased the percentage of E374K mutants (22.86% vs 72.34%, p<0.001) and significantly elevated the frequency of mutations in Sa antigenic site (2.36% vs 23.40%, p<0.001). Future pre-vaccination public health efforts should monitor amino acids of HA and NA of pandemic influenza viruses isolated at exponential and peak phases in areas with high cluster cases. Public Library of Science 2012-02-06 /pmc/articles/PMC3273476/ /pubmed/22328930 http://dx.doi.org/10.1371/journal.pone.0031162 Text en Kao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kao, Chuan-Liang
Chan, Ta-Chien
Tsai, Chu-Han
Chu, Kuan-Ying
Chuang, Shu-Fang
Lee, Chang-Chun
Li, Zheng-Rong Tiger
Wu, Ko-Wen
Chang, Luan-Yin
Shen, Yea-Huei
Huang, Li-Min
Lee, Ping-Ing
Yang, ChingLai
Compans, Richard
Rouse, Barry T.
King, Chwan-Chuen
Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title_full Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title_fullStr Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title_full_unstemmed Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title_short Emerged HA and NA Mutants of the Pandemic Influenza H1N1 Viruses with Increasing Epidemiological Significance in Taipei and Kaohsiung, Taiwan, 2009–10
title_sort emerged ha and na mutants of the pandemic influenza h1n1 viruses with increasing epidemiological significance in taipei and kaohsiung, taiwan, 2009–10
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273476/
https://www.ncbi.nlm.nih.gov/pubmed/22328930
http://dx.doi.org/10.1371/journal.pone.0031162
work_keys_str_mv AT kaochuanliang emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT chantachien emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT tsaichuhan emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT chukuanying emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT chuangshufang emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT leechangchun emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT lizhengrongtiger emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT wukowen emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT changluanyin emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT shenyeahuei emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT huanglimin emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT leepinging emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT yangchinglai emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT compansrichard emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT rousebarryt emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910
AT kingchwanchuen emergedhaandnamutantsofthepandemicinfluenzah1n1viruseswithincreasingepidemiologicalsignificanceintaipeiandkaohsiungtaiwan200910