Cargando…
IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas
Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273483/ https://www.ncbi.nlm.nih.gov/pubmed/22328921 http://dx.doi.org/10.1371/journal.pone.0030729 |
_version_ | 1782222946642690048 |
---|---|
author | Wood, Christopher R. Wang, Zhaohui Diener, Dennis Zones, James Matt Rosenbaum, Joel Umen, James G. |
author_facet | Wood, Christopher R. Wang, Zhaohui Diener, Dennis Zones, James Matt Rosenbaum, Joel Umen, James G. |
author_sort | Wood, Christopher R. |
collection | PubMed |
description | Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division. |
format | Online Article Text |
id | pubmed-3273483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32734832012-02-10 IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas Wood, Christopher R. Wang, Zhaohui Diener, Dennis Zones, James Matt Rosenbaum, Joel Umen, James G. PLoS One Research Article Intraflagellar transport (IFT) proteins are well established as conserved mediators of flagellum/cilium assembly and disassembly. However, data has begun to accumulate in support of IFT protein involvement in other processes elsewhere in the cell. Here, we used synchronous cultures of Chlamydomonas to investigate the temporal patterns of accumulation and localization of IFT proteins during the cell cycle. Their mRNAs showed periodic expression that peaked during S and M phase (S/M). Unlike most proteins that are synthesized continuously during G1 phase, IFT27 and IFT46 levels were found to increase only during S/M phase. During cell division, IFT27, IFT46, IFT72, and IFT139 re-localized from the flagella and basal bodies to the cleavage furrow. IFT27 was further shown to be associated with membrane vesicles in this region. This localization pattern suggests a role for IFT in cell division. Public Library of Science 2012-02-06 /pmc/articles/PMC3273483/ /pubmed/22328921 http://dx.doi.org/10.1371/journal.pone.0030729 Text en Wood et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wood, Christopher R. Wang, Zhaohui Diener, Dennis Zones, James Matt Rosenbaum, Joel Umen, James G. IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title | IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title_full | IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title_fullStr | IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title_full_unstemmed | IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title_short | IFT Proteins Accumulate during Cell Division and Localize to the Cleavage Furrow in Chlamydomonas |
title_sort | ift proteins accumulate during cell division and localize to the cleavage furrow in chlamydomonas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273483/ https://www.ncbi.nlm.nih.gov/pubmed/22328921 http://dx.doi.org/10.1371/journal.pone.0030729 |
work_keys_str_mv | AT woodchristopherr iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas AT wangzhaohui iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas AT dienerdennis iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas AT zonesjamesmatt iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas AT rosenbaumjoel iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas AT umenjamesg iftproteinsaccumulateduringcelldivisionandlocalizetothecleavagefurrowinchlamydomonas |