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A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress

Endoplasmic reticulum (ER) stress is increasingly recognized as an important mechanism in a wide range of diseases including cystic fibrosis, alpha-1 antitrypsin deficiency, Parkinson's and Alzheimer's disease. Therefore, there is an increased need for reliable and quantitative markers for...

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Autores principales: van Schadewijk, Annemarie, van’t Wout, Emily F. A., Stolk, Jan, Hiemstra, Pieter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273559/
https://www.ncbi.nlm.nih.gov/pubmed/22038282
http://dx.doi.org/10.1007/s12192-011-0306-2
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author van Schadewijk, Annemarie
van’t Wout, Emily F. A.
Stolk, Jan
Hiemstra, Pieter S.
author_facet van Schadewijk, Annemarie
van’t Wout, Emily F. A.
Stolk, Jan
Hiemstra, Pieter S.
author_sort van Schadewijk, Annemarie
collection PubMed
description Endoplasmic reticulum (ER) stress is increasingly recognized as an important mechanism in a wide range of diseases including cystic fibrosis, alpha-1 antitrypsin deficiency, Parkinson's and Alzheimer's disease. Therefore, there is an increased need for reliable and quantitative markers for detection of ER stress in human tissues and cells. Accumulation of unfolded or misfolded proteins in the endoplasmic reticulum can cause ER stress, which leads to the activation of the unfolded protein response (UPR). UPR signaling involves splicing of X-box binding protein-1 (XBP1) mRNA, which is frequently used as a marker for ER stress. In most studies, the splicing of the XBP1 mRNA is visualized by gel electrophoresis which is laborious and difficult to quantify. In the present study, we have developed and validated a quantitative real-time RT-PCR method to detect the spliced form of XBP1 mRNA.
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spelling pubmed-32735592012-02-23 A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress van Schadewijk, Annemarie van’t Wout, Emily F. A. Stolk, Jan Hiemstra, Pieter S. Cell Stress Chaperones Short Communication Endoplasmic reticulum (ER) stress is increasingly recognized as an important mechanism in a wide range of diseases including cystic fibrosis, alpha-1 antitrypsin deficiency, Parkinson's and Alzheimer's disease. Therefore, there is an increased need for reliable and quantitative markers for detection of ER stress in human tissues and cells. Accumulation of unfolded or misfolded proteins in the endoplasmic reticulum can cause ER stress, which leads to the activation of the unfolded protein response (UPR). UPR signaling involves splicing of X-box binding protein-1 (XBP1) mRNA, which is frequently used as a marker for ER stress. In most studies, the splicing of the XBP1 mRNA is visualized by gel electrophoresis which is laborious and difficult to quantify. In the present study, we have developed and validated a quantitative real-time RT-PCR method to detect the spliced form of XBP1 mRNA. Springer Netherlands 2011-10-31 2012-03 /pmc/articles/PMC3273559/ /pubmed/22038282 http://dx.doi.org/10.1007/s12192-011-0306-2 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Short Communication
van Schadewijk, Annemarie
van’t Wout, Emily F. A.
Stolk, Jan
Hiemstra, Pieter S.
A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title_full A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title_fullStr A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title_full_unstemmed A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title_short A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress
title_sort quantitative method for detection of spliced x-box binding protein-1 (xbp1) mrna as a measure of endoplasmic reticulum (er) stress
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273559/
https://www.ncbi.nlm.nih.gov/pubmed/22038282
http://dx.doi.org/10.1007/s12192-011-0306-2
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