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Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination

Standard Illumina mate-paired libraries are constructed from 3- to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3–5-kb DNA fragment are not easy to identify and pose problems during mapping and de novo assembly. Longer...

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Detalles Bibliográficos
Autores principales:	Van Nieuwerburgh, Filip, Thompson, Ryan C., Ledesma, Jessica, Deforce, Dieter, Gaasterland, Terry, Ordoukhanian, Phillip, Head, Steven R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273786/ https://www.ncbi.nlm.nih.gov/pubmed/22127871 http://dx.doi.org/10.1093/nar/gkr1000

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