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Construction of repeat-free fluorescence in situ hybridization probes

FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic coverage, a random library is made from BAC clones by whole-genome a...

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Detalles Bibliográficos
Autores principales: Swennenhuis, Joost F., Foulk, Brad, Coumans, Frank A.W., Terstappen, Leon W. M. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273791/
https://www.ncbi.nlm.nih.gov/pubmed/22123742
http://dx.doi.org/10.1093/nar/gkr1123

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