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Illuminating the reaction pathway of the FokI restriction endonuclease by fluorescence resonance energy transfer
The FokI restriction endonuclease is a monomeric protein that recognizes an asymmetric sequence and cleaves both DNA strands at fixed loci downstream of the site. Its single active site is positioned initially near the recognition sequence, distant from its downstream target 13 nucleotides away. Mor...
Autores principales: | Pernstich, Christian, Halford, Stephen E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273807/ https://www.ncbi.nlm.nih.gov/pubmed/21993298 http://dx.doi.org/10.1093/nar/gkr809 |
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