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Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode

In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH(3))(6)](3+), as the...

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Autores principales: Zhong, Guangxian, Liu, Ailin, Xu, Xiongwei, Sun, Zhouliang, Chen, Jinyuan, Wang, Kun, Liu, Qicai, Lin, Xinhua, Lin, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273984/
https://www.ncbi.nlm.nih.gov/pubmed/22334782
http://dx.doi.org/10.2147/IJN.S27794
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author Zhong, Guangxian
Liu, Ailin
Xu, Xiongwei
Sun, Zhouliang
Chen, Jinyuan
Wang, Kun
Liu, Qicai
Lin, Xinhua
Lin, Jianhua
author_facet Zhong, Guangxian
Liu, Ailin
Xu, Xiongwei
Sun, Zhouliang
Chen, Jinyuan
Wang, Kun
Liu, Qicai
Lin, Xinhua
Lin, Jianhua
author_sort Zhong, Guangxian
collection PubMed
description In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH(3))(6)](3+), as the electrochemical indicator. The effect of different frequencies on the real surface area of the nanostructure gold electrode obtained by repetitive square-wave oxidation reduction cycle was investigated. At the optimal frequency of 8000 Hz, the real surface of the developed nanostructure gold electrode was about 42.5 times compared with that of the bare planar gold electrode. The capture probe DNA was immobilized on the nanostructure gold electrode and hybridized with target DNA. Electrochemical signals of hexaamine-ruthenium III bound to the anionic phosphate of DNA strands via electrostatic interactions were measured by chronocoulometry before and after hybridization. The increase of the charges of hexaamine-ruthenium III was observed upon hybridization of the probe with target DNA. Results indicate that this DNA biosensor could detect the femtomole (fM) concentration of the DNA target quantitatively in the range of 50 fM to 250 fM; the detection limit of this DNA biosensor was 5.6 fM (signal to noise = 3). This new biosensor exhibits excellent sensitivity and selectivity and has been used for an assay of polymerase chain reaction (PCR) with a satisfactory result.
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spelling pubmed-32739842012-02-14 Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode Zhong, Guangxian Liu, Ailin Xu, Xiongwei Sun, Zhouliang Chen, Jinyuan Wang, Kun Liu, Qicai Lin, Xinhua Lin, Jianhua Int J Nanomedicine Original Research In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH(3))(6)](3+), as the electrochemical indicator. The effect of different frequencies on the real surface area of the nanostructure gold electrode obtained by repetitive square-wave oxidation reduction cycle was investigated. At the optimal frequency of 8000 Hz, the real surface of the developed nanostructure gold electrode was about 42.5 times compared with that of the bare planar gold electrode. The capture probe DNA was immobilized on the nanostructure gold electrode and hybridized with target DNA. Electrochemical signals of hexaamine-ruthenium III bound to the anionic phosphate of DNA strands via electrostatic interactions were measured by chronocoulometry before and after hybridization. The increase of the charges of hexaamine-ruthenium III was observed upon hybridization of the probe with target DNA. Results indicate that this DNA biosensor could detect the femtomole (fM) concentration of the DNA target quantitatively in the range of 50 fM to 250 fM; the detection limit of this DNA biosensor was 5.6 fM (signal to noise = 3). This new biosensor exhibits excellent sensitivity and selectivity and has been used for an assay of polymerase chain reaction (PCR) with a satisfactory result. Dove Medical Press 2012 2012-02-01 /pmc/articles/PMC3273984/ /pubmed/22334782 http://dx.doi.org/10.2147/IJN.S27794 Text en © 2012 Zhong et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Zhong, Guangxian
Liu, Ailin
Xu, Xiongwei
Sun, Zhouliang
Chen, Jinyuan
Wang, Kun
Liu, Qicai
Lin, Xinhua
Lin, Jianhua
Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title_full Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title_fullStr Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title_full_unstemmed Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title_short Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode
title_sort detection of femtomolar level osteosarcoma-related gene via a chronocoulometric dna biosensor based on nanostructure gold electrode
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273984/
https://www.ncbi.nlm.nih.gov/pubmed/22334782
http://dx.doi.org/10.2147/IJN.S27794
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