Homology-directed Fanconi anemia pathway crosslink repair is dependent on DNA replication

Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand crosslinks (ICLs) in mammalian cells. Cells from Fanconi anemia (FA) patients are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here, we exa...

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Detalles Bibliográficos
Autores principales: Nakanishi, Koji, Cavallo, Francesca, Perrouault, Loïc, Giovannangeli, Carine, Moynahan, Mary Ellen, Barchi, Marco, Brunet, Erika, Jasin, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273992/
https://www.ncbi.nlm.nih.gov/pubmed/21423196
http://dx.doi.org/10.1038/nsmb.2029
Descripción
Sumario:Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand crosslinks (ICLs) in mammalian cells. Cells from Fanconi anemia (FA) patients are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here, we examined ICL-induced HDR using a GFP reporter and observed a profound defect in ICL-induced HDR in FA cells, but only when the reporter could replicate.

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