Cargando…

Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms

The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect...

Descripción completa

Detalles Bibliográficos
Autores principales: Pedrero, María, Campuzano, Susana, Pingarrón, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274141/
https://www.ncbi.nlm.nih.gov/pubmed/22346711
http://dx.doi.org/10.3390/s90705503
_version_ 1782223020255870976
author Pedrero, María
Campuzano, Susana
Pingarrón, José M.
author_facet Pedrero, María
Campuzano, Susana
Pingarrón, José M.
author_sort Pedrero, María
collection PubMed
description The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered.
format Online
Article
Text
id pubmed-3274141
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-32741412012-02-15 Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms Pedrero, María Campuzano, Susana Pingarrón, José M. Sensors (Basel) Review The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered. Molecular Diversity Preservation International (MDPI) 2009-07-13 /pmc/articles/PMC3274141/ /pubmed/22346711 http://dx.doi.org/10.3390/s90705503 Text en © 2009 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Pedrero, María
Campuzano, Susana
Pingarrón, José M.
Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title_full Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title_fullStr Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title_full_unstemmed Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title_short Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms
title_sort electroanalytical sensors and devices for multiplexed detection of foodborne pathogen microorganisms
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274141/
https://www.ncbi.nlm.nih.gov/pubmed/22346711
http://dx.doi.org/10.3390/s90705503
work_keys_str_mv AT pedreromaria electroanalyticalsensorsanddevicesformultiplexeddetectionoffoodbornepathogenmicroorganisms
AT campuzanosusana electroanalyticalsensorsanddevicesformultiplexeddetectionoffoodbornepathogenmicroorganisms
AT pingarronjosem electroanalyticalsensorsanddevicesformultiplexeddetectionoffoodbornepathogenmicroorganisms