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Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin
A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274204/ https://www.ncbi.nlm.nih.gov/pubmed/22319276 http://dx.doi.org/10.3390/s100402851 |
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author | Ismail, Fatma Adeloju, Samuel B. |
author_facet | Ismail, Fatma Adeloju, Samuel B. |
author_sort | Ismail, Fatma |
collection | PubMed |
description | A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO(3), and 3 mM penicillin with an applied current density of 0.8 mA/cm(2) and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 μM for penicillin and achieved a minimum detectable concentration of 0.3 μM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm. |
format | Online Article Text |
id | pubmed-3274204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32742042012-02-08 Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin Ismail, Fatma Adeloju, Samuel B. Sensors (Basel) Article A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO(3), and 3 mM penicillin with an applied current density of 0.8 mA/cm(2) and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3–283 μM for penicillin and achieved a minimum detectable concentration of 0.3 μM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ± 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ≥20 ppm. Molecular Diversity Preservation International (MDPI) 2010-03-29 /pmc/articles/PMC3274204/ /pubmed/22319276 http://dx.doi.org/10.3390/s100402851 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Ismail, Fatma Adeloju, Samuel B. Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title_full | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title_fullStr | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title_full_unstemmed | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title_short | Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin |
title_sort | galvanostatic entrapment of penicillinase into polytyramine films and its utilization for the potentiometric determination of penicillin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274204/ https://www.ncbi.nlm.nih.gov/pubmed/22319276 http://dx.doi.org/10.3390/s100402851 |
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