A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro

We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro. The instrument features eight culture wells; each containing three gold sensors around a common silver/silver chloride reference elec...

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Detalles Bibliográficos
Autores principales: Adlam, Daman J., Woolley, David E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274243/
https://www.ncbi.nlm.nih.gov/pubmed/22319322
http://dx.doi.org/10.3390/s100403732
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author Adlam, Daman J.
Woolley, David E.
author_facet Adlam, Daman J.
Woolley, David E.
author_sort Adlam, Daman J.
collection PubMed
description We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro. The instrument features eight culture wells; each containing three gold sensors around a common silver/silver chloride reference electrode, prepared using screen-printed conductive inks. The potential applications of the device were demonstrated by monitoring rheumatoid synovial fibroblasts (RSF) and HepG2 hepatocarcinoma cells in response to chemical and biological treatments. This technology could provide an alternative to conventional end-point assays used in the fields of chemotherapy, toxicology and drug discovery.
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spelling pubmed-32742432012-02-08 A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro Adlam, Daman J. Woolley, David E. Sensors (Basel) Article We report the development of a multiwell biosensor for detecting changes in the electrochemical open circuit potential (OCP) generated by viable human cells in vitro. The instrument features eight culture wells; each containing three gold sensors around a common silver/silver chloride reference electrode, prepared using screen-printed conductive inks. The potential applications of the device were demonstrated by monitoring rheumatoid synovial fibroblasts (RSF) and HepG2 hepatocarcinoma cells in response to chemical and biological treatments. This technology could provide an alternative to conventional end-point assays used in the fields of chemotherapy, toxicology and drug discovery. Molecular Diversity Preservation International (MDPI) 2010-04-13 /pmc/articles/PMC3274243/ /pubmed/22319322 http://dx.doi.org/10.3390/s100403732 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Adlam, Daman J.
Woolley, David E.
A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title_full A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title_fullStr A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title_full_unstemmed A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title_short A Multiwell Electrochemical Biosensor for Real-Time Monitoring of the Behavioural Changes of Cells in Vitro
title_sort multiwell electrochemical biosensor for real-time monitoring of the behavioural changes of cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274243/
https://www.ncbi.nlm.nih.gov/pubmed/22319322
http://dx.doi.org/10.3390/s100403732
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AT adlamdamanj multiwellelectrochemicalbiosensorforrealtimemonitoringofthebehaviouralchangesofcellsinvitro
AT woolleydavide multiwellelectrochemicalbiosensorforrealtimemonitoringofthebehaviouralchangesofcellsinvitro

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