A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg(2+) and Sybr Green I

We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg(2+) due to the formation of a thymine-Hg(2+)-thymine (T-Hg(2+)-T)...

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Detalles Bibliográficos
Autores principales: Xu, Hui, Gao, Shuli, Liu, Quanwen, Pan, Dun, Wang, Lihua, Ren, Shuzhen, Ding, Min, Chen, Jingwen, Liu, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274280/
https://www.ncbi.nlm.nih.gov/pubmed/22346638
http://dx.doi.org/10.3390/s111110187

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