Cargando…

A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α

We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Jiefang, Guo, Aitao, Zhang, Zhaoyang, Guo, Lei, Xie, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274296/
https://www.ncbi.nlm.nih.gov/pubmed/22346654
http://dx.doi.org/10.3390/s111110490
_version_ 1782223054614560768
author Sun, Jiefang
Guo, Aitao
Zhang, Zhaoyang
Guo, Lei
Xie, Jianwei
author_facet Sun, Jiefang
Guo, Aitao
Zhang, Zhaoyang
Guo, Lei
Xie, Jianwei
author_sort Sun, Jiefang
collection PubMed
description We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours.
format Online
Article
Text
id pubmed-3274296
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-32742962012-02-15 A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α Sun, Jiefang Guo, Aitao Zhang, Zhaoyang Guo, Lei Xie, Jianwei Sensors (Basel) Article We present here a novel conjugated aptamer-gold nanoparticle (Apt-AuNPs) fluorescent probe and its application for specific detection of recombinant human erythropoietin-α (rHuEPO-α). In this nanobiosensor, 12 nm AuNPs function as both a nano-scaffold and a nano-quencher (fluorescent energy acceptor), on the surface of which the complementary sequences are linked (as cODN-AuNPs) and pre-hybridized with carboxymethylfluorescein (FAM)-labeled anti-rHuEPO-α aptamers. Upon target protein binding, the aptamers can be released from the AuNP surface and the fluorescence signal is restored. Key variables such as the length of linker, the hybridization site and length have been designed and optimized. Full performance evaluation including sensitivity, linear range and interference substances are also described. This nanobiosensor provides a promising approach for a simple and direct quantification of rHuEPO-α concentrations as low as 0.92 nM within a few hours. Molecular Diversity Preservation International (MDPI) 2011-11-03 /pmc/articles/PMC3274296/ /pubmed/22346654 http://dx.doi.org/10.3390/s111110490 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Sun, Jiefang
Guo, Aitao
Zhang, Zhaoyang
Guo, Lei
Xie, Jianwei
A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title_full A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title_fullStr A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title_full_unstemmed A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title_short A Conjugated Aptamer-Gold Nanoparticle Fluorescent Probe for Highly Sensitive Detection of rHuEPO-α
title_sort conjugated aptamer-gold nanoparticle fluorescent probe for highly sensitive detection of rhuepo-α
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274296/
https://www.ncbi.nlm.nih.gov/pubmed/22346654
http://dx.doi.org/10.3390/s111110490
work_keys_str_mv AT sunjiefang aconjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT guoaitao aconjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT zhangzhaoyang aconjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT guolei aconjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT xiejianwei aconjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT sunjiefang conjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT guoaitao conjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT zhangzhaoyang conjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT guolei conjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa
AT xiejianwei conjugatedaptamergoldnanoparticlefluorescentprobeforhighlysensitivedetectionofrhuepoa