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Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination

BACKGROUND: Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge. METHODS: We recruited healthy PM+ and PM-...

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Autores principales: Walther, Brigitte, Miles, David JC, Waight, Pauline, Palmero, Melba S, Ojuola, Olubukola, Touray, Ebrima S, Whittle, Hilton, van der Sande, Marianne, Crozier, Sarah, Flanagan, Katie L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274427/
https://www.ncbi.nlm.nih.gov/pubmed/22243970
http://dx.doi.org/10.1186/1471-2334-12-6
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author Walther, Brigitte
Miles, David JC
Waight, Pauline
Palmero, Melba S
Ojuola, Olubukola
Touray, Ebrima S
Whittle, Hilton
van der Sande, Marianne
Crozier, Sarah
Flanagan, Katie L
author_facet Walther, Brigitte
Miles, David JC
Waight, Pauline
Palmero, Melba S
Ojuola, Olubukola
Touray, Ebrima S
Whittle, Hilton
van der Sande, Marianne
Crozier, Sarah
Flanagan, Katie L
author_sort Walther, Brigitte
collection PubMed
description BACKGROUND: Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge. METHODS: We recruited healthy PM+ and PM- infants at birth. At six and 12 months, we stimulated PBMCs with tuberculin purified protein derivative (PPD) and compared expression of CD154, IL-2 and IFNγ by CD4 T-cells to a negative control using flow cytometry. We measured the length, weight and head circumference at birth and 12 months. RESULTS: IL-2 and CD154 expression were low in both groups at both timepoints, without discernable differences. Expression of IFNγ was similarly low at 6 months but by 12 months, the median response was higher in PM- than PM + infants (p = 0.026). The PM+ infants also had a lower weight (p = 0.032) and head circumference (p = 0.041) at 12 months, indicating lower growth rates. At birth, the size and weight of the PM+ and PM- infants were equivalent. By 12 months, the PM+ infants had a lower weight and head circumference than the PM- infants. CONCLUSIONS: Placental malaria was associated with reduced immune responses 12 months after immune challenge in infants apparently healthy at birth.
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spelling pubmed-32744272012-02-08 Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination Walther, Brigitte Miles, David JC Waight, Pauline Palmero, Melba S Ojuola, Olubukola Touray, Ebrima S Whittle, Hilton van der Sande, Marianne Crozier, Sarah Flanagan, Katie L BMC Infect Dis Research Article BACKGROUND: Placental malaria (PM) is associated with prenatal malaise, but many PM+ infants are born without symptoms. As malaria has powerful immunomodulatory effects, we tested the hypothesis that PM predicts reduced T-cell responses to vaccine challenge. METHODS: We recruited healthy PM+ and PM- infants at birth. At six and 12 months, we stimulated PBMCs with tuberculin purified protein derivative (PPD) and compared expression of CD154, IL-2 and IFNγ by CD4 T-cells to a negative control using flow cytometry. We measured the length, weight and head circumference at birth and 12 months. RESULTS: IL-2 and CD154 expression were low in both groups at both timepoints, without discernable differences. Expression of IFNγ was similarly low at 6 months but by 12 months, the median response was higher in PM- than PM + infants (p = 0.026). The PM+ infants also had a lower weight (p = 0.032) and head circumference (p = 0.041) at 12 months, indicating lower growth rates. At birth, the size and weight of the PM+ and PM- infants were equivalent. By 12 months, the PM+ infants had a lower weight and head circumference than the PM- infants. CONCLUSIONS: Placental malaria was associated with reduced immune responses 12 months after immune challenge in infants apparently healthy at birth. BioMed Central 2012-01-14 /pmc/articles/PMC3274427/ /pubmed/22243970 http://dx.doi.org/10.1186/1471-2334-12-6 Text en Copyright ©2012 Walther et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Walther, Brigitte
Miles, David JC
Waight, Pauline
Palmero, Melba S
Ojuola, Olubukola
Touray, Ebrima S
Whittle, Hilton
van der Sande, Marianne
Crozier, Sarah
Flanagan, Katie L
Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title_full Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title_fullStr Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title_full_unstemmed Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title_short Placental malaria is associated with attenuated CD4 T-cell responses to tuberculin PPD 12 months after BCG vaccination
title_sort placental malaria is associated with attenuated cd4 t-cell responses to tuberculin ppd 12 months after bcg vaccination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274427/
https://www.ncbi.nlm.nih.gov/pubmed/22243970
http://dx.doi.org/10.1186/1471-2334-12-6
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