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5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells

BACKGROUND: Adipose tissue is a source of multipotent adult stem cells and it has the ability to differentiate into several types of cell lineages such as neuron cells, osteogenic cells and adipogenic cells. Several reports have shown adipose-derived stem cells (ASCs) have the ability to undergo car...

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Autores principales: Wan Safwani, Wan Kamarul Zaman, Makpol, Suzana, Sathapan, Somasundaram, Chua, Kien Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274438/
https://www.ncbi.nlm.nih.gov/pubmed/22221649
http://dx.doi.org/10.1186/1477-5751-11-3
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author Wan Safwani, Wan Kamarul Zaman
Makpol, Suzana
Sathapan, Somasundaram
Chua, Kien Hui
author_facet Wan Safwani, Wan Kamarul Zaman
Makpol, Suzana
Sathapan, Somasundaram
Chua, Kien Hui
author_sort Wan Safwani, Wan Kamarul Zaman
collection PubMed
description BACKGROUND: Adipose tissue is a source of multipotent adult stem cells and it has the ability to differentiate into several types of cell lineages such as neuron cells, osteogenic cells and adipogenic cells. Several reports have shown adipose-derived stem cells (ASCs) have the ability to undergo cardiomyogenesis. Studies have shown 5-azacytidine can successfully drive stem cells such as bone marrow derived stem cells to differentiate into cardiomyogenic cells. Therefore, in this study, we investigated the effect 5-azacytidine on the cardiogenic ability of ASCs. METHODS: The cardiogenic potential of ASCs was analysed by studying the morphological changes after induction, the changes in the cardiogenic genes expression i.e. GATA4, MLC-2v, MLC-2a, NKX2.5, β-MHC, α-MHC, Atrial natriuretic peptide (ANP), Connexin 43, Cardiac Troponin C, Cardiac Troponin I and myocyte enhancer factor (MEF2C) and the changes of embryonic stem cells genes expression at P5 and P10 using quantitative PCR. RESULTS: Our results showed that the induced ASCs did not show significant morphological difference compared to the non-induced ASCs. While quantitative PCR data indicated that most cardiogenic genes and stemness genes expression level decreased after induction at P5 and P10. CONCLUSION: 5-azacytidine is insufficient for the cardiogenic induction of the ASCs.
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spelling pubmed-32744382012-02-08 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells Wan Safwani, Wan Kamarul Zaman Makpol, Suzana Sathapan, Somasundaram Chua, Kien Hui J Negat Results Biomed Research BACKGROUND: Adipose tissue is a source of multipotent adult stem cells and it has the ability to differentiate into several types of cell lineages such as neuron cells, osteogenic cells and adipogenic cells. Several reports have shown adipose-derived stem cells (ASCs) have the ability to undergo cardiomyogenesis. Studies have shown 5-azacytidine can successfully drive stem cells such as bone marrow derived stem cells to differentiate into cardiomyogenic cells. Therefore, in this study, we investigated the effect 5-azacytidine on the cardiogenic ability of ASCs. METHODS: The cardiogenic potential of ASCs was analysed by studying the morphological changes after induction, the changes in the cardiogenic genes expression i.e. GATA4, MLC-2v, MLC-2a, NKX2.5, β-MHC, α-MHC, Atrial natriuretic peptide (ANP), Connexin 43, Cardiac Troponin C, Cardiac Troponin I and myocyte enhancer factor (MEF2C) and the changes of embryonic stem cells genes expression at P5 and P10 using quantitative PCR. RESULTS: Our results showed that the induced ASCs did not show significant morphological difference compared to the non-induced ASCs. While quantitative PCR data indicated that most cardiogenic genes and stemness genes expression level decreased after induction at P5 and P10. CONCLUSION: 5-azacytidine is insufficient for the cardiogenic induction of the ASCs. BioMed Central 2012-01-06 /pmc/articles/PMC3274438/ /pubmed/22221649 http://dx.doi.org/10.1186/1477-5751-11-3 Text en Copyright ©2012 Wan Safwani et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wan Safwani, Wan Kamarul Zaman
Makpol, Suzana
Sathapan, Somasundaram
Chua, Kien Hui
5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title_full 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title_fullStr 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title_full_unstemmed 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title_short 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
title_sort 5-azacytidine is insufficient for cardiogenesis in human adipose-derived stem cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274438/
https://www.ncbi.nlm.nih.gov/pubmed/22221649
http://dx.doi.org/10.1186/1477-5751-11-3
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