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Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence

Tomato is an important crop and hence there is a great interest in understanding the genetic basis of its flowering. Several genes have been identified by mutations and we constructed a set of novel double mutants to understand how these genes interact to shape the inflorescence. It was previously s...

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Autores principales: Thouet, Johanna, Quinet, Muriel, Lutts, Stanley, Kinet, Jean-Marie, Périlleux, Claire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274538/
https://www.ncbi.nlm.nih.gov/pubmed/22347436
http://dx.doi.org/10.1371/journal.pone.0031096
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author Thouet, Johanna
Quinet, Muriel
Lutts, Stanley
Kinet, Jean-Marie
Périlleux, Claire
author_facet Thouet, Johanna
Quinet, Muriel
Lutts, Stanley
Kinet, Jean-Marie
Périlleux, Claire
author_sort Thouet, Johanna
collection PubMed
description Tomato is an important crop and hence there is a great interest in understanding the genetic basis of its flowering. Several genes have been identified by mutations and we constructed a set of novel double mutants to understand how these genes interact to shape the inflorescence. It was previously suggested that the branching of the tomato inflorescence depends on the gradual transition from inflorescence meristem (IM) to flower meristem (FM): the extension of this time window allows IM to branch, as seen in the compound inflorescence (s) and falsiflora (fa) mutants that are impaired in FM maturation. We report here that JOINTLESS (J), which encodes a MADS-box protein of the same clade than SHORT VEGETATIVE PHASE (SVP) and AGAMOUS LIKE 24 (AGL24) in Arabidopsis, interferes with this timing and delays FM maturation, therefore promoting IM fate. This was inferred from the fact that j mutation suppresses the high branching inflorescence phenotype of s and fa mutants and was further supported by the expression pattern of J, which is expressed more strongly in IM than in FM. Most interestingly, FA - the orthologue of the Arabidopsis LEAFY (LFY) gene - shows the complementary expression pattern and is more active in FM than in IM. Loss of J function causes premature termination of flower formation in the inflorescence and its reversion to a vegetative program. This phenotype is enhanced in the absence of systemic florigenic protein, encoded by the SINGLE FLOWER TRUSS (SFT) gene, the tomato orthologue of FLOWERING LOCUS T (FT). These results suggest that the formation of an inflorescence in tomato requires the interaction of J and a target of SFT in the meristem, for repressing FA activity and FM fate in the IM.
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spelling pubmed-32745382012-02-15 Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence Thouet, Johanna Quinet, Muriel Lutts, Stanley Kinet, Jean-Marie Périlleux, Claire PLoS One Research Article Tomato is an important crop and hence there is a great interest in understanding the genetic basis of its flowering. Several genes have been identified by mutations and we constructed a set of novel double mutants to understand how these genes interact to shape the inflorescence. It was previously suggested that the branching of the tomato inflorescence depends on the gradual transition from inflorescence meristem (IM) to flower meristem (FM): the extension of this time window allows IM to branch, as seen in the compound inflorescence (s) and falsiflora (fa) mutants that are impaired in FM maturation. We report here that JOINTLESS (J), which encodes a MADS-box protein of the same clade than SHORT VEGETATIVE PHASE (SVP) and AGAMOUS LIKE 24 (AGL24) in Arabidopsis, interferes with this timing and delays FM maturation, therefore promoting IM fate. This was inferred from the fact that j mutation suppresses the high branching inflorescence phenotype of s and fa mutants and was further supported by the expression pattern of J, which is expressed more strongly in IM than in FM. Most interestingly, FA - the orthologue of the Arabidopsis LEAFY (LFY) gene - shows the complementary expression pattern and is more active in FM than in IM. Loss of J function causes premature termination of flower formation in the inflorescence and its reversion to a vegetative program. This phenotype is enhanced in the absence of systemic florigenic protein, encoded by the SINGLE FLOWER TRUSS (SFT) gene, the tomato orthologue of FLOWERING LOCUS T (FT). These results suggest that the formation of an inflorescence in tomato requires the interaction of J and a target of SFT in the meristem, for repressing FA activity and FM fate in the IM. Public Library of Science 2012-02-07 /pmc/articles/PMC3274538/ /pubmed/22347436 http://dx.doi.org/10.1371/journal.pone.0031096 Text en Thouet et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Thouet, Johanna
Quinet, Muriel
Lutts, Stanley
Kinet, Jean-Marie
Périlleux, Claire
Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title_full Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title_fullStr Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title_full_unstemmed Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title_short Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence
title_sort repression of floral meristem fate is crucial in shaping tomato inflorescence
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274538/
https://www.ncbi.nlm.nih.gov/pubmed/22347436
http://dx.doi.org/10.1371/journal.pone.0031096
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