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In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization
A broth for the screening of group B streptococcal (GBS) carriage during pregnancy is about to be introduced. Simulating conditions in everyday practice, we have compared the sensitivity of this Granada tube broth (GT) with that of classical Amies transport medium (AT) in vitro. A total of 1,485 GT...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274678/ https://www.ncbi.nlm.nih.gov/pubmed/21698495 http://dx.doi.org/10.1007/s10096-011-1317-8 |
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author | te Witt, R. Oostvogel, P. M. Yahiaoui, R. Wu, Y. van Belkum, A. Muller, A. E. |
author_facet | te Witt, R. Oostvogel, P. M. Yahiaoui, R. Wu, Y. van Belkum, A. Muller, A. E. |
author_sort | te Witt, R. |
collection | PubMed |
description | A broth for the screening of group B streptococcal (GBS) carriage during pregnancy is about to be introduced. Simulating conditions in everyday practice, we have compared the sensitivity of this Granada tube broth (GT) with that of classical Amies transport medium (AT) in vitro. A total of 1,485 GT and 1,485 AT were tested with 33 well-characterized GBS strains in three different concentrations, five different incubation times, and three different temperatures. After initial incubation at room temperature (RT) or 4°C, GT were placed at 37°C. GT were scored for the presence of orange pigment. GT and AT were subcultured on blood agar (BA). Pigment was observed in 98% of GT incubated at 37°C. GBS could be cultured in 91%, 73%, and 55% of GT incubated at 37°C, RT, or 4°C, respectively. For AT, these percentages were only 20% at 37°C, 52% at RT, and 59% at 4°C. When GT initially incubated at RT or 4°C were subsequently incubated at 37°C, the sensitivity improved significantly. We conclude that GT is a more sensitive GBS transport and culture medium than the conventional method, especially for low inocula and prolonged transport/incubation times. GT does not exclude the presence of GBS, and should always be incubated at 37°C and subcultured on solid agar for optimal sensitivity. |
format | Online Article Text |
id | pubmed-3274678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-32746782012-02-21 In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization te Witt, R. Oostvogel, P. M. Yahiaoui, R. Wu, Y. van Belkum, A. Muller, A. E. Eur J Clin Microbiol Infect Dis Article A broth for the screening of group B streptococcal (GBS) carriage during pregnancy is about to be introduced. Simulating conditions in everyday practice, we have compared the sensitivity of this Granada tube broth (GT) with that of classical Amies transport medium (AT) in vitro. A total of 1,485 GT and 1,485 AT were tested with 33 well-characterized GBS strains in three different concentrations, five different incubation times, and three different temperatures. After initial incubation at room temperature (RT) or 4°C, GT were placed at 37°C. GT were scored for the presence of orange pigment. GT and AT were subcultured on blood agar (BA). Pigment was observed in 98% of GT incubated at 37°C. GBS could be cultured in 91%, 73%, and 55% of GT incubated at 37°C, RT, or 4°C, respectively. For AT, these percentages were only 20% at 37°C, 52% at RT, and 59% at 4°C. When GT initially incubated at RT or 4°C were subsequently incubated at 37°C, the sensitivity improved significantly. We conclude that GT is a more sensitive GBS transport and culture medium than the conventional method, especially for low inocula and prolonged transport/incubation times. GT does not exclude the presence of GBS, and should always be incubated at 37°C and subcultured on solid agar for optimal sensitivity. Springer-Verlag 2011-06-23 2012 /pmc/articles/PMC3274678/ /pubmed/21698495 http://dx.doi.org/10.1007/s10096-011-1317-8 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article te Witt, R. Oostvogel, P. M. Yahiaoui, R. Wu, Y. van Belkum, A. Muller, A. E. In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title | In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title_full | In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title_fullStr | In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title_full_unstemmed | In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title_short | In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization |
title_sort | in vitro evaluation of the performance of granada selective enrichment broth for the detection of group b streptococcal colonization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274678/ https://www.ncbi.nlm.nih.gov/pubmed/21698495 http://dx.doi.org/10.1007/s10096-011-1317-8 |
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