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Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility
BACKGROUND: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutop...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275521/ https://www.ncbi.nlm.nih.gov/pubmed/22233680 http://dx.doi.org/10.1186/1477-7827-10-1 |
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author | Szczepańska, Malgorzata Wirstlein, Przemyslaw Skrzypczak, Jana Jagodziński, Paweł P |
author_facet | Szczepańska, Malgorzata Wirstlein, Przemyslaw Skrzypczak, Jana Jagodziński, Paweł P |
author_sort | Szczepańska, Malgorzata |
collection | PubMed |
description | BACKGROUND: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women. RESULTS: There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women. CONCLUSIONS: We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis. |
format | Online Article Text |
id | pubmed-3275521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32755212012-02-09 Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility Szczepańska, Malgorzata Wirstlein, Przemyslaw Skrzypczak, Jana Jagodziński, Paweł P Reprod Biol Endocrinol Research BACKGROUND: A decrease in HOXA11 expression in eutopic mid-secretory endometrium has been found in women with endometriosis-associated infertility. METHODS: Using Real-time quantitative PCR (RQ-PCR) and western blotting analysis we studied the HOXA11 transcript and protein levels in mid-luteal eutopic endometrium from eighteen infertile women with minimal endometriosis, sixteen healthy fertile women and sixteen infertile women with fallopian tubal occlusion from the Polish population. We also evaluated transcript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in these groups of women. RESULTS: There were significantly lower levels of HOXA11 transcripts (p = 0.003, p = 0.041) and protein (p = 0.004, p = 0.001) in women with endometriosis as compared to fertile women and infertile women with tubal occlusion. Moreover, we found significantly higher methylation levels of the CpG region in the first exon of HOXA11 in infertile women with endometriosis compared with fertile women (p < 0.001) and infertile women with tubal occlusion (p < 0.001). We also observed significantly increased levels of DNMT3A transcript in women with endometriosis than fertile women (p = 0.044) and infertile women with tubal occlusion (p = 0.047). However, we did not observe significant differences in DNMT1 and DNMT3B transcript levels between these investigated groups of women. CONCLUSIONS: We confirmed that reduced HOXA11 expression may contribute to endometriosis-associated infertility. Moreover, we found that DNA hypermethylation can be one of the possible molecular mechanisms causing a decrease in HOXA11 expression in the eutopic mid-secretory endometrium in infertile women with endometriosis. BioMed Central 2012-01-10 /pmc/articles/PMC3275521/ /pubmed/22233680 http://dx.doi.org/10.1186/1477-7827-10-1 Text en Copyright ©2012 Szczepańska et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Szczepańska, Malgorzata Wirstlein, Przemyslaw Skrzypczak, Jana Jagodziński, Paweł P Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title | Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title_full | Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title_fullStr | Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title_full_unstemmed | Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title_short | Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
title_sort | expression of hoxa11 in the mid-luteal endometrium from women with endometriosis-associated infertility |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275521/ https://www.ncbi.nlm.nih.gov/pubmed/22233680 http://dx.doi.org/10.1186/1477-7827-10-1 |
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