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Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis

BACKGROUND: Endogenous uveitis is a chronic inflammatory eye disease of human, which frequently leads to blindness. Experimental autoimmune uveoretinitis (EAU) is an animal disease model of human endogenous uveitis and can be induced in susceptible animals by immunization with retinal antigens. EAU...

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Autores principales: Oh, Keunhee, Kim, Yon Su, Lee, Dong-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275710/
https://www.ncbi.nlm.nih.gov/pubmed/22346781
http://dx.doi.org/10.4110/in.2011.11.6.399
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author Oh, Keunhee
Kim, Yon Su
Lee, Dong-Sup
author_facet Oh, Keunhee
Kim, Yon Su
Lee, Dong-Sup
author_sort Oh, Keunhee
collection PubMed
description BACKGROUND: Endogenous uveitis is a chronic inflammatory eye disease of human, which frequently leads to blindness. Experimental autoimmune uveoretinitis (EAU) is an animal disease model of human endogenous uveitis and can be induced in susceptible animals by immunization with retinal antigens. EAU resembles the key immunological characteristics of human disease in that both are CD4(+) T-cell mediated diseases. Dendritic cells (DCs) are specialized antigen-presenting cells that are uniquely capable of activating naïve T cells. Regulation of immune responses through modulation of DCs has thus been tried extensively. Recently our group reported that donor strain-derived immature DC pretreatment successfully controlled the adverse immune response during allogeneic transplantation. METHODS: EAU was induced by immunization with human interphotoreceptor retinoid-binding protein (IRBP) peptide(1-20). Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF. RESULTS: In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state. Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation. CONCLUSION: Application of iBMDC prepared according to the protocol of this study would provide an important treatment modality for the autoimmune diseases and transplantation rejection.
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spelling pubmed-32757102012-02-16 Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis Oh, Keunhee Kim, Yon Su Lee, Dong-Sup Immune Netw Original Article BACKGROUND: Endogenous uveitis is a chronic inflammatory eye disease of human, which frequently leads to blindness. Experimental autoimmune uveoretinitis (EAU) is an animal disease model of human endogenous uveitis and can be induced in susceptible animals by immunization with retinal antigens. EAU resembles the key immunological characteristics of human disease in that both are CD4(+) T-cell mediated diseases. Dendritic cells (DCs) are specialized antigen-presenting cells that are uniquely capable of activating naïve T cells. Regulation of immune responses through modulation of DCs has thus been tried extensively. Recently our group reported that donor strain-derived immature DC pretreatment successfully controlled the adverse immune response during allogeneic transplantation. METHODS: EAU was induced by immunization with human interphotoreceptor retinoid-binding protein (IRBP) peptide(1-20). Dendritic cells were differentiated from bone marrow in the presence of recombinant GM-CSF. RESULTS: In this study, we used paraformaldehyde-fixed bone marrow-derived DCs to maintain them in an immature state. Pretreatment with fixed immature DCs, but not fixed mature DCs, ameliorated the disease progression of EAU by inhibiting uveitogenic CD4(+) T cell activation and differentiation. CONCLUSION: Application of iBMDC prepared according to the protocol of this study would provide an important treatment modality for the autoimmune diseases and transplantation rejection. The Korean Association of Immunologists 2011-12 2011-12-31 /pmc/articles/PMC3275710/ /pubmed/22346781 http://dx.doi.org/10.4110/in.2011.11.6.399 Text en Copyright © 2011 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Oh, Keunhee
Kim, Yon Su
Lee, Dong-Sup
Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title_full Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title_fullStr Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title_full_unstemmed Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title_short Maturation-Resistant Dendritic Cells Ameliorate Experimental Autoimmune Uveoretinitis
title_sort maturation-resistant dendritic cells ameliorate experimental autoimmune uveoretinitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275710/
https://www.ncbi.nlm.nih.gov/pubmed/22346781
http://dx.doi.org/10.4110/in.2011.11.6.399
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