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The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations

Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the...

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Autores principales: Bass, Chris, Nikou, Dimitra, Vontas, John, Donnelly, Martin J., Williamson, Martin S., Field, Linda M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276000/
https://www.ncbi.nlm.nih.gov/pubmed/22347668
http://dx.doi.org/10.4061/2010/190434
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author Bass, Chris
Nikou, Dimitra
Vontas, John
Donnelly, Martin J.
Williamson, Martin S.
Field, Linda M.
author_facet Bass, Chris
Nikou, Dimitra
Vontas, John
Donnelly, Martin J.
Williamson, Martin S.
Field, Linda M.
author_sort Bass, Chris
collection PubMed
description Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the Innovative Vector Control Consortium (IVCC), have developed a suite of high-throughput assays based on a single “closed-tube” platform that collectively comprise the “Vector Population Monitoring Tool” (VPMT). The VPMT can be used to screen mosquito disease vector populations for a number of traits including Anopheles gambiae s.l. and Anopheles funestus species identification, detection of infection with Plasmodium parasites, and identification of insecticide resistance mechanisms. In this paper we focus on the Anopheles-specific assays that comprise the VPMT and include details of a new assay for resistance todieldrin Rdl detection. The application of these tools, general and specific guidelines on their use based on field testing in Africa, and plans for further development are discussed.
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spelling pubmed-32760002012-02-16 The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations Bass, Chris Nikou, Dimitra Vontas, John Donnelly, Martin J. Williamson, Martin S. Field, Linda M. Malar Res Treat Review Article Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the Innovative Vector Control Consortium (IVCC), have developed a suite of high-throughput assays based on a single “closed-tube” platform that collectively comprise the “Vector Population Monitoring Tool” (VPMT). The VPMT can be used to screen mosquito disease vector populations for a number of traits including Anopheles gambiae s.l. and Anopheles funestus species identification, detection of infection with Plasmodium parasites, and identification of insecticide resistance mechanisms. In this paper we focus on the Anopheles-specific assays that comprise the VPMT and include details of a new assay for resistance todieldrin Rdl detection. The application of these tools, general and specific guidelines on their use based on field testing in Africa, and plans for further development are discussed. SAGE-Hindawi Access to Research 2010 2010-12-22 /pmc/articles/PMC3276000/ /pubmed/22347668 http://dx.doi.org/10.4061/2010/190434 Text en Copyright © 2010 Chris Bass et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Bass, Chris
Nikou, Dimitra
Vontas, John
Donnelly, Martin J.
Williamson, Martin S.
Field, Linda M.
The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title_full The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title_fullStr The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title_full_unstemmed The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title_short The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
title_sort vector population monitoring tool (vpmt): high-throughput dna-based diagnostics for the monitoring of mosquito vector populations
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276000/
https://www.ncbi.nlm.nih.gov/pubmed/22347668
http://dx.doi.org/10.4061/2010/190434
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