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High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit

Heat stress can cause severe crop yield losses by impairing reproductive development. However, the underlying mechanisms are poorly understood. We examined patterns of carbon allocation and activities of sucrose cleavage enzymes in heat-tolerant (HT) and -sensitive (HS) tomato (Solanum lycopersicum...

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Autores principales: Li, Zhimiao, Palmer, William M., Martin, Antony P., Wang, Rongqing, Rainsford, Frederick, Jin, Ye, Patrick, John W., Yang, Yuejian, Ruan, Yong-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276082/
https://www.ncbi.nlm.nih.gov/pubmed/22105847
http://dx.doi.org/10.1093/jxb/err329
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author Li, Zhimiao
Palmer, William M.
Martin, Antony P.
Wang, Rongqing
Rainsford, Frederick
Jin, Ye
Patrick, John W.
Yang, Yuejian
Ruan, Yong-Ling
author_facet Li, Zhimiao
Palmer, William M.
Martin, Antony P.
Wang, Rongqing
Rainsford, Frederick
Jin, Ye
Patrick, John W.
Yang, Yuejian
Ruan, Yong-Ling
author_sort Li, Zhimiao
collection PubMed
description Heat stress can cause severe crop yield losses by impairing reproductive development. However, the underlying mechanisms are poorly understood. We examined patterns of carbon allocation and activities of sucrose cleavage enzymes in heat-tolerant (HT) and -sensitive (HS) tomato (Solanum lycopersicum L.) lines subjected to normal (control) and heat stress temperatures. At the control temperature of 25/20 °C (day/night) the HT line exhibited higher cell wall invertase (CWIN) activity in flowers and young fruits and partitioned more sucrose to fruits but less to vegetative tissues as compared to the HS line, independent of leaf photosynthetic capacity. Upon 2-, 4-, or 24-h exposure to day or night temperatures of 5 °C or more above 25/20 °C, cell wall (CWIN) and vacuolar invertases (VIN), but not sucrose synthase (SuSy), activities in young fruit of the HT line were significantly higher than those of the HS line. The HT line had a higher level of transcript of a CWIN gene, Lin7, in 5-day fruit than the HS line under control and heat stress temperatures. Interestingly, heat induced transcription of an invertase inhibitor gene, INVINH1, but reduced its protein abundance. Transcript levels of LePLDa1, encoding phospholipase D, which degrades cell membranes, was less in the HT line than in the HS line after exposure to heat stress. The data indicate that high invertase activity of, and increased sucrose import into, young tomato fruit could contribute to their heat tolerance through increasing sink strength and sugar signalling activities, possibly regulating a programmed cell death pathway.
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spelling pubmed-32760822012-02-09 High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit Li, Zhimiao Palmer, William M. Martin, Antony P. Wang, Rongqing Rainsford, Frederick Jin, Ye Patrick, John W. Yang, Yuejian Ruan, Yong-Ling J Exp Bot Research Papers Heat stress can cause severe crop yield losses by impairing reproductive development. However, the underlying mechanisms are poorly understood. We examined patterns of carbon allocation and activities of sucrose cleavage enzymes in heat-tolerant (HT) and -sensitive (HS) tomato (Solanum lycopersicum L.) lines subjected to normal (control) and heat stress temperatures. At the control temperature of 25/20 °C (day/night) the HT line exhibited higher cell wall invertase (CWIN) activity in flowers and young fruits and partitioned more sucrose to fruits but less to vegetative tissues as compared to the HS line, independent of leaf photosynthetic capacity. Upon 2-, 4-, or 24-h exposure to day or night temperatures of 5 °C or more above 25/20 °C, cell wall (CWIN) and vacuolar invertases (VIN), but not sucrose synthase (SuSy), activities in young fruit of the HT line were significantly higher than those of the HS line. The HT line had a higher level of transcript of a CWIN gene, Lin7, in 5-day fruit than the HS line under control and heat stress temperatures. Interestingly, heat induced transcription of an invertase inhibitor gene, INVINH1, but reduced its protein abundance. Transcript levels of LePLDa1, encoding phospholipase D, which degrades cell membranes, was less in the HT line than in the HS line after exposure to heat stress. The data indicate that high invertase activity of, and increased sucrose import into, young tomato fruit could contribute to their heat tolerance through increasing sink strength and sugar signalling activities, possibly regulating a programmed cell death pathway. Oxford University Press 2012-02 2011-11-21 /pmc/articles/PMC3276082/ /pubmed/22105847 http://dx.doi.org/10.1093/jxb/err329 Text en © 2011 The Author(s). http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Li, Zhimiao
Palmer, William M.
Martin, Antony P.
Wang, Rongqing
Rainsford, Frederick
Jin, Ye
Patrick, John W.
Yang, Yuejian
Ruan, Yong-Ling
High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title_full High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title_fullStr High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title_full_unstemmed High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title_short High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
title_sort high invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276082/
https://www.ncbi.nlm.nih.gov/pubmed/22105847
http://dx.doi.org/10.1093/jxb/err329
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