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Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)

Interspecific hybridization has a much greater effect than chromosome doubling on gene expression; however, the associations between homeologous gene expression changes and polyhaploidization had rarely been addressed. In this study, cDNA–single strand conformation polymorphism analysis was applied...

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Autores principales: Wang, Jiali, Liu, Dongcheng, Guo, Xiaoli, Yang, Wenlong, Wang, XiuJie, Zhan, Kehui, Zhang, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276123/
https://www.ncbi.nlm.nih.gov/pubmed/22384315
http://dx.doi.org/10.1534/g3.111.000091
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author Wang, Jiali
Liu, Dongcheng
Guo, Xiaoli
Yang, Wenlong
Wang, XiuJie
Zhan, Kehui
Zhang, Aimin
author_facet Wang, Jiali
Liu, Dongcheng
Guo, Xiaoli
Yang, Wenlong
Wang, XiuJie
Zhan, Kehui
Zhang, Aimin
author_sort Wang, Jiali
collection PubMed
description Interspecific hybridization has a much greater effect than chromosome doubling on gene expression; however, the associations between homeologous gene expression changes and polyhaploidization had rarely been addressed. In this study, cDNA–single strand conformation polymorphism analysis was applied to measure the expression of 30 homeologous transcripts in naturally occurring haploid (ABD, 2n = 21) and its polyploid maternal parent Yumai 21A (AABBDD, 2n = 42) in wheat. Only one gene (TC251989) showed preferentially silenced homoeoalleles in haploids. Further analyses of 24 single-copy genes known to be silenced in the root and/or leaf also found no evidence of homeologous silencing in 1-month-old haploids and two ESTs (BF484100 and BF473379) exhibit different expression patterns between 4-month-old haploids and hexaploids. Global analysis of the gene expression patterns using the Affymetrix GeneChip showed that of the 55,052 genes probed, only about 0.11% in the shoots and 0.25% in the roots were activated by polyhaploidization. The results demonstrate that activation and silencing of homoeoalleles were not widespread in haploid seedlings.
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spelling pubmed-32761232012-03-01 Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.) Wang, Jiali Liu, Dongcheng Guo, Xiaoli Yang, Wenlong Wang, XiuJie Zhan, Kehui Zhang, Aimin G3 (Bethesda) Investigation Interspecific hybridization has a much greater effect than chromosome doubling on gene expression; however, the associations between homeologous gene expression changes and polyhaploidization had rarely been addressed. In this study, cDNA–single strand conformation polymorphism analysis was applied to measure the expression of 30 homeologous transcripts in naturally occurring haploid (ABD, 2n = 21) and its polyploid maternal parent Yumai 21A (AABBDD, 2n = 42) in wheat. Only one gene (TC251989) showed preferentially silenced homoeoalleles in haploids. Further analyses of 24 single-copy genes known to be silenced in the root and/or leaf also found no evidence of homeologous silencing in 1-month-old haploids and two ESTs (BF484100 and BF473379) exhibit different expression patterns between 4-month-old haploids and hexaploids. Global analysis of the gene expression patterns using the Affymetrix GeneChip showed that of the 55,052 genes probed, only about 0.11% in the shoots and 0.25% in the roots were activated by polyhaploidization. The results demonstrate that activation and silencing of homoeoalleles were not widespread in haploid seedlings. Genetics Society of America 2011-06-01 /pmc/articles/PMC3276123/ /pubmed/22384315 http://dx.doi.org/10.1534/g3.111.000091 Text en Copyright © 2011 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Wang, Jiali
Liu, Dongcheng
Guo, Xiaoli
Yang, Wenlong
Wang, XiuJie
Zhan, Kehui
Zhang, Aimin
Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title_full Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title_fullStr Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title_full_unstemmed Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title_short Variability of Gene Expression After Polyhaploidization in Wheat (Triticum aestivum L.)
title_sort variability of gene expression after polyhaploidization in wheat (triticum aestivum l.)
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276123/
https://www.ncbi.nlm.nih.gov/pubmed/22384315
http://dx.doi.org/10.1534/g3.111.000091
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