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Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation

PURPOSE: The aim of this prospective study was to evaluate whether blending two kinds of biomaterials, chitosan and polycaprolactone (PCL), can be used as scaffold and carrier for growth and differentiation of corneal endothelial cells (CECs). METHODS: A transparent, biocompatible carrier with cultu...

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Autores principales: Wang, Tsung-Jen, Wang, I-Jong, Lu, Jui-Nan, Young, Tai-Horng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276373/
https://www.ncbi.nlm.nih.gov/pubmed/22328821
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author Wang, Tsung-Jen
Wang, I-Jong
Lu, Jui-Nan
Young, Tai-Horng
author_facet Wang, Tsung-Jen
Wang, I-Jong
Lu, Jui-Nan
Young, Tai-Horng
author_sort Wang, Tsung-Jen
collection PubMed
description PURPOSE: The aim of this prospective study was to evaluate whether blending two kinds of biomaterials, chitosan and polycaprolactone (PCL), can be used as scaffold and carrier for growth and differentiation of corneal endothelial cells (CECs). METHODS: A transparent, biocompatible carrier with cultured CECs on scaffold would be a perfect replacement graft. In the initial part of experiment, for essential and biocompatible test, chitosan and PCL were evaluated respectively and blended in various proportions by coating. In the later part of this study, for evaluation of potential application, homogenous solutions of 25%, 50%, and 75% PCL compositions were attempted to structure blend membranes. RESULTS: Chitosan, PCL 25, PCL 50, and PCL 75 blends could maintain transparency of culturing substrata. BCECs were found to be reached confluence successfully after 7 days on PCL 25, PCL 50, and PCL 75. The expression of tight junction and extracellular matrix protein were observed as well. Alternatively, only PCL 25 could make blend membrane with enough strength during preparation for carrier in culture. On this blend membrane, the growth pattern and phenotype of BCECs could be observed well. CONCLUSIONS: A ratio of 75:25 (chitosan:PCL) blends showed enough mechanical properties as well as suitable support for cellular activity in cultivating BCECs. Thus, a novel methodology of biodegradable carrier from chitosan and PCL has potential to be a good replacement scaffold for raising CECs for clinical transplantation.
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spelling pubmed-32763732012-02-10 Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation Wang, Tsung-Jen Wang, I-Jong Lu, Jui-Nan Young, Tai-Horng Mol Vis Research Article PURPOSE: The aim of this prospective study was to evaluate whether blending two kinds of biomaterials, chitosan and polycaprolactone (PCL), can be used as scaffold and carrier for growth and differentiation of corneal endothelial cells (CECs). METHODS: A transparent, biocompatible carrier with cultured CECs on scaffold would be a perfect replacement graft. In the initial part of experiment, for essential and biocompatible test, chitosan and PCL were evaluated respectively and blended in various proportions by coating. In the later part of this study, for evaluation of potential application, homogenous solutions of 25%, 50%, and 75% PCL compositions were attempted to structure blend membranes. RESULTS: Chitosan, PCL 25, PCL 50, and PCL 75 blends could maintain transparency of culturing substrata. BCECs were found to be reached confluence successfully after 7 days on PCL 25, PCL 50, and PCL 75. The expression of tight junction and extracellular matrix protein were observed as well. Alternatively, only PCL 25 could make blend membrane with enough strength during preparation for carrier in culture. On this blend membrane, the growth pattern and phenotype of BCECs could be observed well. CONCLUSIONS: A ratio of 75:25 (chitosan:PCL) blends showed enough mechanical properties as well as suitable support for cellular activity in cultivating BCECs. Thus, a novel methodology of biodegradable carrier from chitosan and PCL has potential to be a good replacement scaffold for raising CECs for clinical transplantation. Molecular Vision 2012-01-31 /pmc/articles/PMC3276373/ /pubmed/22328821 Text en Copyright © 2012 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Tsung-Jen
Wang, I-Jong
Lu, Jui-Nan
Young, Tai-Horng
Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title_full Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title_fullStr Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title_full_unstemmed Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title_short Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
title_sort novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276373/
https://www.ncbi.nlm.nih.gov/pubmed/22328821
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