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Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes

BACKGROUND: The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little...

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Autores principales: Ettorre, Michele, Lorenzetto, Erika, Laperchia, Claudia, Baiguera, Cristina, Branca, Caterina, Benarese, Manuela, Spano, PierFranco, Pizzi, Marina, Buffelli, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276509/
https://www.ncbi.nlm.nih.gov/pubmed/22347480
http://dx.doi.org/10.1371/journal.pone.0031451
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author Ettorre, Michele
Lorenzetto, Erika
Laperchia, Claudia
Baiguera, Cristina
Branca, Caterina
Benarese, Manuela
Spano, PierFranco
Pizzi, Marina
Buffelli, Mario
author_facet Ettorre, Michele
Lorenzetto, Erika
Laperchia, Claudia
Baiguera, Cristina
Branca, Caterina
Benarese, Manuela
Spano, PierFranco
Pizzi, Marina
Buffelli, Mario
author_sort Ettorre, Michele
collection PubMed
description BACKGROUND: The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known. METHODS AND FINDINGS: To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction. CONCLUSIONS: These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures.
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spelling pubmed-32765092012-02-15 Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes Ettorre, Michele Lorenzetto, Erika Laperchia, Claudia Baiguera, Cristina Branca, Caterina Benarese, Manuela Spano, PierFranco Pizzi, Marina Buffelli, Mario PLoS One Research Article BACKGROUND: The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known. METHODS AND FINDINGS: To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction. CONCLUSIONS: These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures. Public Library of Science 2012-02-09 /pmc/articles/PMC3276509/ /pubmed/22347480 http://dx.doi.org/10.1371/journal.pone.0031451 Text en Ettorre et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ettorre, Michele
Lorenzetto, Erika
Laperchia, Claudia
Baiguera, Cristina
Branca, Caterina
Benarese, Manuela
Spano, PierFranco
Pizzi, Marina
Buffelli, Mario
Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title_full Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title_fullStr Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title_full_unstemmed Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title_short Glutamatergic Neurons Induce Expression of Functional Glutamatergic Synapses in Primary Myotubes
title_sort glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276509/
https://www.ncbi.nlm.nih.gov/pubmed/22347480
http://dx.doi.org/10.1371/journal.pone.0031451
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