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Rootstock-regulated gene expression patterns associated with fire blight resistance in apple

BACKGROUND: Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including resistance to pathogens such as Erwinia amylovora, which causes fire blight, the most serious bacterial disease of apple. T...

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Autores principales: Jensen, Philip J, Halbrendt, Noemi, Fazio, Gennaro, Makalowska, Izabela, Altman, Naomi, Praul, Craig, Maximova, Siela N, Ngugi, Henry K, Crassweller, Robert M, Travis, James W, McNellis, Timothy W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277459/
https://www.ncbi.nlm.nih.gov/pubmed/22229964
http://dx.doi.org/10.1186/1471-2164-13-9
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author Jensen, Philip J
Halbrendt, Noemi
Fazio, Gennaro
Makalowska, Izabela
Altman, Naomi
Praul, Craig
Maximova, Siela N
Ngugi, Henry K
Crassweller, Robert M
Travis, James W
McNellis, Timothy W
author_facet Jensen, Philip J
Halbrendt, Noemi
Fazio, Gennaro
Makalowska, Izabela
Altman, Naomi
Praul, Craig
Maximova, Siela N
Ngugi, Henry K
Crassweller, Robert M
Travis, James W
McNellis, Timothy W
author_sort Jensen, Philip J
collection PubMed
description BACKGROUND: Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including resistance to pathogens such as Erwinia amylovora, which causes fire blight, the most serious bacterial disease of apple. The purpose of the present study was to quantify rootstock-mediated differences in scion fire blight susceptibility and to identify transcripts in the scion whose expression levels correlated with this response. RESULTS: Rootstock influence on scion fire blight resistance was quantified by inoculating three-year old, orchard-grown apple trees, consisting of 'Gala' scions grafted to a range of rootstocks, with E. amylovora. Disease severity was measured by the extent of shoot necrosis over time. 'Gala' scions grafted to G.30 or MM.111 rootstocks showed the lowest rates of necrosis, while 'Gala' on M.27 and B.9 showed the highest rates of necrosis. 'Gala' scions on M.7, S.4 or M.9F56 had intermediate necrosis rates. Using an apple DNA microarray representing 55,230 unique transcripts, gene expression patterns were compared in healthy, un-inoculated, greenhouse-grown 'Gala' scions on the same series of rootstocks. We identified 690 transcripts whose steady-state expression levels correlated with the degree of fire blight susceptibility of the scion/rootstock combinations. Transcripts known to be differentially expressed during E. amylovora infection were disproportionately represented among these transcripts. A second-generation apple microarray representing 26,000 transcripts was developed and was used to test these correlations in an orchard-grown population of trees segregating for fire blight resistance. Of the 690 transcripts originally identified using the first-generation array, 39 had expression levels that correlated with fire blight resistance in the breeding population. CONCLUSIONS: Rootstocks had significant effects on the fire blight susceptibility of 'Gala' scions, and rootstock-regulated gene expression patterns could be correlated with differences in susceptibility. The results suggest a relationship between rootstock-regulated fire blight susceptibility and sorbitol dehydrogenase, phenylpropanoid metabolism, protein processing in the endoplasmic reticulum, and endocytosis, among others. This study illustrates the utility of our rootstock-regulated gene expression data sets for candidate trait-associated gene data mining.
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spelling pubmed-32774592012-02-11 Rootstock-regulated gene expression patterns associated with fire blight resistance in apple Jensen, Philip J Halbrendt, Noemi Fazio, Gennaro Makalowska, Izabela Altman, Naomi Praul, Craig Maximova, Siela N Ngugi, Henry K Crassweller, Robert M Travis, James W McNellis, Timothy W BMC Genomics Research Article BACKGROUND: Desirable apple varieties are clonally propagated by grafting vegetative scions onto rootstocks. Rootstocks influence many phenotypic traits of the scion, including resistance to pathogens such as Erwinia amylovora, which causes fire blight, the most serious bacterial disease of apple. The purpose of the present study was to quantify rootstock-mediated differences in scion fire blight susceptibility and to identify transcripts in the scion whose expression levels correlated with this response. RESULTS: Rootstock influence on scion fire blight resistance was quantified by inoculating three-year old, orchard-grown apple trees, consisting of 'Gala' scions grafted to a range of rootstocks, with E. amylovora. Disease severity was measured by the extent of shoot necrosis over time. 'Gala' scions grafted to G.30 or MM.111 rootstocks showed the lowest rates of necrosis, while 'Gala' on M.27 and B.9 showed the highest rates of necrosis. 'Gala' scions on M.7, S.4 or M.9F56 had intermediate necrosis rates. Using an apple DNA microarray representing 55,230 unique transcripts, gene expression patterns were compared in healthy, un-inoculated, greenhouse-grown 'Gala' scions on the same series of rootstocks. We identified 690 transcripts whose steady-state expression levels correlated with the degree of fire blight susceptibility of the scion/rootstock combinations. Transcripts known to be differentially expressed during E. amylovora infection were disproportionately represented among these transcripts. A second-generation apple microarray representing 26,000 transcripts was developed and was used to test these correlations in an orchard-grown population of trees segregating for fire blight resistance. Of the 690 transcripts originally identified using the first-generation array, 39 had expression levels that correlated with fire blight resistance in the breeding population. CONCLUSIONS: Rootstocks had significant effects on the fire blight susceptibility of 'Gala' scions, and rootstock-regulated gene expression patterns could be correlated with differences in susceptibility. The results suggest a relationship between rootstock-regulated fire blight susceptibility and sorbitol dehydrogenase, phenylpropanoid metabolism, protein processing in the endoplasmic reticulum, and endocytosis, among others. This study illustrates the utility of our rootstock-regulated gene expression data sets for candidate trait-associated gene data mining. BioMed Central 2012-01-09 /pmc/articles/PMC3277459/ /pubmed/22229964 http://dx.doi.org/10.1186/1471-2164-13-9 Text en Copyright ©2012 Jensen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jensen, Philip J
Halbrendt, Noemi
Fazio, Gennaro
Makalowska, Izabela
Altman, Naomi
Praul, Craig
Maximova, Siela N
Ngugi, Henry K
Crassweller, Robert M
Travis, James W
McNellis, Timothy W
Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title_full Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title_fullStr Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title_full_unstemmed Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title_short Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
title_sort rootstock-regulated gene expression patterns associated with fire blight resistance in apple
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277459/
https://www.ncbi.nlm.nih.gov/pubmed/22229964
http://dx.doi.org/10.1186/1471-2164-13-9
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