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MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation
The Polycomb Group (PcG) of chromatin modifiers regulates pluripotency and differentiation. Mammalian genomes encode multiple homologs of the Polycomb repressive complex 1 (PRC1) components, including five orthologs of the Drosophila Polycomb protein (Cbx2, Cbx4, Cbx6, Cbx7, and Cbx8). We have ident...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277884/ https://www.ncbi.nlm.nih.gov/pubmed/22226354 http://dx.doi.org/10.1016/j.stem.2011.12.004 |
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author | O'Loghlen, Ana Muñoz-Cabello, Ana M. Gaspar-Maia, Alexandre Wu, Hsan-Au Banito, Ana Kunowska, Natalia Racek, Tomas Pemberton, Helen N. Beolchi, Patrizia Lavial, Fabrice Masui, Osamu Vermeulen, Michiel Carroll, Thomas Graumann, Johannes Heard, Edith Dillon, Niall Azuara, Veronique Snijders, Ambrosius P. Peters, Gordon Bernstein, Emily Gil, Jesus |
author_facet | O'Loghlen, Ana Muñoz-Cabello, Ana M. Gaspar-Maia, Alexandre Wu, Hsan-Au Banito, Ana Kunowska, Natalia Racek, Tomas Pemberton, Helen N. Beolchi, Patrizia Lavial, Fabrice Masui, Osamu Vermeulen, Michiel Carroll, Thomas Graumann, Johannes Heard, Edith Dillon, Niall Azuara, Veronique Snijders, Ambrosius P. Peters, Gordon Bernstein, Emily Gil, Jesus |
author_sort | O'Loghlen, Ana |
collection | PubMed |
description | The Polycomb Group (PcG) of chromatin modifiers regulates pluripotency and differentiation. Mammalian genomes encode multiple homologs of the Polycomb repressive complex 1 (PRC1) components, including five orthologs of the Drosophila Polycomb protein (Cbx2, Cbx4, Cbx6, Cbx7, and Cbx8). We have identified Cbx7 as the primary Polycomb ortholog of PRC1 complexes in embryonic stem cells (ESCs). The expression of Cbx7 is downregulated during ESC differentiation, preceding the upregulation of Cbx2, Cbx4, and Cbx8, which are directly repressed by Cbx7. Ectopic expression of Cbx7 inhibits differentiation and X chromosome inactivation and enhances ESC self-renewal. Conversely, Cbx7 knockdown induces differentiation and derepresses lineage-specific markers. In a functional screen, we identified the miR-125 and miR-181 families as regulators of Cbx7 that are induced during ESC differentiation. Ectopic expression of these miRNAs accelerates ESC differentiation via regulation of Cbx7. These observations establish a critical role for Cbx7 and its regulatory miRNAs in determining pluripotency. |
format | Online Article Text |
id | pubmed-3277884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32778842012-02-24 MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation O'Loghlen, Ana Muñoz-Cabello, Ana M. Gaspar-Maia, Alexandre Wu, Hsan-Au Banito, Ana Kunowska, Natalia Racek, Tomas Pemberton, Helen N. Beolchi, Patrizia Lavial, Fabrice Masui, Osamu Vermeulen, Michiel Carroll, Thomas Graumann, Johannes Heard, Edith Dillon, Niall Azuara, Veronique Snijders, Ambrosius P. Peters, Gordon Bernstein, Emily Gil, Jesus Cell Stem Cell Article The Polycomb Group (PcG) of chromatin modifiers regulates pluripotency and differentiation. Mammalian genomes encode multiple homologs of the Polycomb repressive complex 1 (PRC1) components, including five orthologs of the Drosophila Polycomb protein (Cbx2, Cbx4, Cbx6, Cbx7, and Cbx8). We have identified Cbx7 as the primary Polycomb ortholog of PRC1 complexes in embryonic stem cells (ESCs). The expression of Cbx7 is downregulated during ESC differentiation, preceding the upregulation of Cbx2, Cbx4, and Cbx8, which are directly repressed by Cbx7. Ectopic expression of Cbx7 inhibits differentiation and X chromosome inactivation and enhances ESC self-renewal. Conversely, Cbx7 knockdown induces differentiation and derepresses lineage-specific markers. In a functional screen, we identified the miR-125 and miR-181 families as regulators of Cbx7 that are induced during ESC differentiation. Ectopic expression of these miRNAs accelerates ESC differentiation via regulation of Cbx7. These observations establish a critical role for Cbx7 and its regulatory miRNAs in determining pluripotency. Cell Press 2012-01-06 /pmc/articles/PMC3277884/ /pubmed/22226354 http://dx.doi.org/10.1016/j.stem.2011.12.004 Text en © 2012 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/4.0/ Open Access under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) license |
spellingShingle | Article O'Loghlen, Ana Muñoz-Cabello, Ana M. Gaspar-Maia, Alexandre Wu, Hsan-Au Banito, Ana Kunowska, Natalia Racek, Tomas Pemberton, Helen N. Beolchi, Patrizia Lavial, Fabrice Masui, Osamu Vermeulen, Michiel Carroll, Thomas Graumann, Johannes Heard, Edith Dillon, Niall Azuara, Veronique Snijders, Ambrosius P. Peters, Gordon Bernstein, Emily Gil, Jesus MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title | MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title_full | MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title_fullStr | MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title_full_unstemmed | MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title_short | MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC Differentiation |
title_sort | microrna regulation of cbx7 mediates a switch of polycomb orthologs during esc differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277884/ https://www.ncbi.nlm.nih.gov/pubmed/22226354 http://dx.doi.org/10.1016/j.stem.2011.12.004 |
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