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Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278405/ https://www.ncbi.nlm.nih.gov/pubmed/22348029 http://dx.doi.org/10.1371/journal.pone.0030904 |
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author | Thuret, Sandrine Thallmair, Michaela Horky, Laura L. Gage, Fred H. |
author_facet | Thuret, Sandrine Thallmair, Michaela Horky, Laura L. Gage, Fred H. |
author_sort | Thuret, Sandrine |
collection | PubMed |
description | Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord injury (SCI) in MRL/MpJ mice. A control group (C57BL/6 mice) and MRL/MpJ mice underwent a dorsal hemisection at T9 (thoracic vertebra 9). Our data show that MRL/MpJ mice recovered motor function significantly faster and more completely. We observed enhanced regeneration of the corticospinal tract (CST). Furthermore, we observed a reduced astrocytic response and fewer micro-cavities at the injury site, which appear to create a more growth-permissive environment for the injured axons. Our data suggest that the reduced astrocytic response is in part due to a lower lesion-induced increase of cell proliferation post-SCI, and a reduced astrocytic differentiation of the proliferating cells. Interestingly, we also found an increased number of proliferating microglia, which could be involved in the MRL/MpJ spinal cord repair mechanisms. Finally, to evaluate the molecular basis of faster spinal cord repair, we examined the difference in gene expression changes in MRL/MpJ and C57BL/6 mice after SCI. Our microarray data support our histological findings and reveal a transcriptional profile associated with a more efficient spinal cord repair in MRL/MpJ mice. |
format | Online Article Text |
id | pubmed-3278405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32784052012-02-17 Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection Thuret, Sandrine Thallmair, Michaela Horky, Laura L. Gage, Fred H. PLoS One Research Article Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord injury (SCI) in MRL/MpJ mice. A control group (C57BL/6 mice) and MRL/MpJ mice underwent a dorsal hemisection at T9 (thoracic vertebra 9). Our data show that MRL/MpJ mice recovered motor function significantly faster and more completely. We observed enhanced regeneration of the corticospinal tract (CST). Furthermore, we observed a reduced astrocytic response and fewer micro-cavities at the injury site, which appear to create a more growth-permissive environment for the injured axons. Our data suggest that the reduced astrocytic response is in part due to a lower lesion-induced increase of cell proliferation post-SCI, and a reduced astrocytic differentiation of the proliferating cells. Interestingly, we also found an increased number of proliferating microglia, which could be involved in the MRL/MpJ spinal cord repair mechanisms. Finally, to evaluate the molecular basis of faster spinal cord repair, we examined the difference in gene expression changes in MRL/MpJ and C57BL/6 mice after SCI. Our microarray data support our histological findings and reveal a transcriptional profile associated with a more efficient spinal cord repair in MRL/MpJ mice. Public Library of Science 2012-02-13 /pmc/articles/PMC3278405/ /pubmed/22348029 http://dx.doi.org/10.1371/journal.pone.0030904 Text en Thuret et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Thuret, Sandrine Thallmair, Michaela Horky, Laura L. Gage, Fred H. Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title | Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title_full | Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title_fullStr | Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title_full_unstemmed | Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title_short | Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection |
title_sort | enhanced functional recovery in mrl/mpj mice after spinal cord dorsal hemisection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278405/ https://www.ncbi.nlm.nih.gov/pubmed/22348029 http://dx.doi.org/10.1371/journal.pone.0030904 |
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