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Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection

Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord i...

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Autores principales: Thuret, Sandrine, Thallmair, Michaela, Horky, Laura L., Gage, Fred H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278405/
https://www.ncbi.nlm.nih.gov/pubmed/22348029
http://dx.doi.org/10.1371/journal.pone.0030904
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author Thuret, Sandrine
Thallmair, Michaela
Horky, Laura L.
Gage, Fred H.
author_facet Thuret, Sandrine
Thallmair, Michaela
Horky, Laura L.
Gage, Fred H.
author_sort Thuret, Sandrine
collection PubMed
description Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord injury (SCI) in MRL/MpJ mice. A control group (C57BL/6 mice) and MRL/MpJ mice underwent a dorsal hemisection at T9 (thoracic vertebra 9). Our data show that MRL/MpJ mice recovered motor function significantly faster and more completely. We observed enhanced regeneration of the corticospinal tract (CST). Furthermore, we observed a reduced astrocytic response and fewer micro-cavities at the injury site, which appear to create a more growth-permissive environment for the injured axons. Our data suggest that the reduced astrocytic response is in part due to a lower lesion-induced increase of cell proliferation post-SCI, and a reduced astrocytic differentiation of the proliferating cells. Interestingly, we also found an increased number of proliferating microglia, which could be involved in the MRL/MpJ spinal cord repair mechanisms. Finally, to evaluate the molecular basis of faster spinal cord repair, we examined the difference in gene expression changes in MRL/MpJ and C57BL/6 mice after SCI. Our microarray data support our histological findings and reveal a transcriptional profile associated with a more efficient spinal cord repair in MRL/MpJ mice.
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spelling pubmed-32784052012-02-17 Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection Thuret, Sandrine Thallmair, Michaela Horky, Laura L. Gage, Fred H. PLoS One Research Article Adult MRL/MpJ mice have been shown to possess unique regeneration capabilities. They are able to heal an ear-punched hole or an injured heart with normal tissue architecture and without scar formation. Here we present functional and histological evidence for enhanced recovery following spinal cord injury (SCI) in MRL/MpJ mice. A control group (C57BL/6 mice) and MRL/MpJ mice underwent a dorsal hemisection at T9 (thoracic vertebra 9). Our data show that MRL/MpJ mice recovered motor function significantly faster and more completely. We observed enhanced regeneration of the corticospinal tract (CST). Furthermore, we observed a reduced astrocytic response and fewer micro-cavities at the injury site, which appear to create a more growth-permissive environment for the injured axons. Our data suggest that the reduced astrocytic response is in part due to a lower lesion-induced increase of cell proliferation post-SCI, and a reduced astrocytic differentiation of the proliferating cells. Interestingly, we also found an increased number of proliferating microglia, which could be involved in the MRL/MpJ spinal cord repair mechanisms. Finally, to evaluate the molecular basis of faster spinal cord repair, we examined the difference in gene expression changes in MRL/MpJ and C57BL/6 mice after SCI. Our microarray data support our histological findings and reveal a transcriptional profile associated with a more efficient spinal cord repair in MRL/MpJ mice. Public Library of Science 2012-02-13 /pmc/articles/PMC3278405/ /pubmed/22348029 http://dx.doi.org/10.1371/journal.pone.0030904 Text en Thuret et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Thuret, Sandrine
Thallmair, Michaela
Horky, Laura L.
Gage, Fred H.
Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title_full Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title_fullStr Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title_full_unstemmed Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title_short Enhanced Functional Recovery in MRL/MpJ Mice after Spinal Cord Dorsal Hemisection
title_sort enhanced functional recovery in mrl/mpj mice after spinal cord dorsal hemisection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278405/
https://www.ncbi.nlm.nih.gov/pubmed/22348029
http://dx.doi.org/10.1371/journal.pone.0030904
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