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Aptamers and Their Biological Applications
Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternati...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279232/ https://www.ncbi.nlm.nih.gov/pubmed/22368488 http://dx.doi.org/10.3390/s120100612 |
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author | Song, Kyung-Mi Lee, Seonghwan Ban, Changill |
author_facet | Song, Kyung-Mi Lee, Seonghwan Ban, Changill |
author_sort | Song, Kyung-Mi |
collection | PubMed |
description | Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment. |
format | Online Article Text |
id | pubmed-3279232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32792322012-02-24 Aptamers and Their Biological Applications Song, Kyung-Mi Lee, Seonghwan Ban, Changill Sensors (Basel) Review Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment. Molecular Diversity Preservation International (MDPI) 2012-01-09 /pmc/articles/PMC3279232/ /pubmed/22368488 http://dx.doi.org/10.3390/s120100612 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Song, Kyung-Mi Lee, Seonghwan Ban, Changill Aptamers and Their Biological Applications |
title | Aptamers and Their Biological Applications |
title_full | Aptamers and Their Biological Applications |
title_fullStr | Aptamers and Their Biological Applications |
title_full_unstemmed | Aptamers and Their Biological Applications |
title_short | Aptamers and Their Biological Applications |
title_sort | aptamers and their biological applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279232/ https://www.ncbi.nlm.nih.gov/pubmed/22368488 http://dx.doi.org/10.3390/s120100612 |
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