Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML)...

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Autores principales: Jain, Sonal, Dass, Jasmita, Pati, Hara Prasad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Università Cattolica del Sacro Cuore 2012
Materias:
Case Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279321/
https://www.ncbi.nlm.nih.gov/pubmed/22348188
http://dx.doi.org/10.4084/MJHID.2012.006
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author Jain, Sonal
Dass, Jasmita
Pati, Hara Prasad
author_facet Jain, Sonal
Dass, Jasmita
Pati, Hara Prasad
author_sort Jain, Sonal
collection PubMed
description High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.
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spelling pubmed-32793212012-02-17 Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report Jain, Sonal Dass, Jasmita Pati, Hara Prasad Mediterr J Hematol Infect Dis Case Reports High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC. Università Cattolica del Sacro Cuore 2012-01-21 /pmc/articles/PMC3279321/ /pubmed/22348188 http://dx.doi.org/10.4084/MJHID.2012.006 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Reports
Jain, Sonal
Dass, Jasmita
Pati, Hara Prasad
Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title_full Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title_fullStr Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title_full_unstemmed Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title_short Transfusion Associated Peak in Hb HPLC Chromatogram – a Case Report
title_sort transfusion associated peak in hb hplc chromatogram – a case report
topic Case Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279321/
https://www.ncbi.nlm.nih.gov/pubmed/22348188
http://dx.doi.org/10.4084/MJHID.2012.006
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AT dassjasmita transfusionassociatedpeakinhbhplcchromatogramacasereport
AT patiharaprasad transfusionassociatedpeakinhbhplcchromatogramacasereport

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