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The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes

Upon transcription, histones can either detach from DNA or transfer behind the polymerase through a process believed to involve template looping. The details governing nucleosomal fate during transcription are not well understood. Our atomic force microscopy images of RNA polymerase II-nucleosome co...

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Detalles Bibliográficos
Autores principales: Bintu, Lacramioara, Kopaczynska, Marta, Hodges, Courtney, Lubkowska, Lucyna, Kashlev, Mikhail, Bustamante, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279329/
https://www.ncbi.nlm.nih.gov/pubmed/22081017
http://dx.doi.org/10.1038/nsmb.2164
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author Bintu, Lacramioara
Kopaczynska, Marta
Hodges, Courtney
Lubkowska, Lucyna
Kashlev, Mikhail
Bustamante, Carlos
author_facet Bintu, Lacramioara
Kopaczynska, Marta
Hodges, Courtney
Lubkowska, Lucyna
Kashlev, Mikhail
Bustamante, Carlos
author_sort Bintu, Lacramioara
collection PubMed
description Upon transcription, histones can either detach from DNA or transfer behind the polymerase through a process believed to involve template looping. The details governing nucleosomal fate during transcription are not well understood. Our atomic force microscopy images of RNA polymerase II-nucleosome complexes confirm the presence of looped transcriptional intermediates and provide mechanistic insight into the histone-transfer process via the distribution of transcribed nucleosome positions. Significantly, we find that a fraction of the transcribed nucleosomes are remodeled to hexasomes, and that this fraction depends on the transcription elongation rate. A simple model involving the kinetic competition between transcription elongation, histone transfer, and histone-histone dissociation quantitatively rationalizes our observations and unifies results obtained with other polymerases. Factors affecting the relative magnitude of these processes provide the physical basis for nucleosomal fate during transcription and, therefore, for the regulation of gene expression.
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spelling pubmed-32793292012-06-01 The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes Bintu, Lacramioara Kopaczynska, Marta Hodges, Courtney Lubkowska, Lucyna Kashlev, Mikhail Bustamante, Carlos Nat Struct Mol Biol Article Upon transcription, histones can either detach from DNA or transfer behind the polymerase through a process believed to involve template looping. The details governing nucleosomal fate during transcription are not well understood. Our atomic force microscopy images of RNA polymerase II-nucleosome complexes confirm the presence of looped transcriptional intermediates and provide mechanistic insight into the histone-transfer process via the distribution of transcribed nucleosome positions. Significantly, we find that a fraction of the transcribed nucleosomes are remodeled to hexasomes, and that this fraction depends on the transcription elongation rate. A simple model involving the kinetic competition between transcription elongation, histone transfer, and histone-histone dissociation quantitatively rationalizes our observations and unifies results obtained with other polymerases. Factors affecting the relative magnitude of these processes provide the physical basis for nucleosomal fate during transcription and, therefore, for the regulation of gene expression. 2011-11-13 /pmc/articles/PMC3279329/ /pubmed/22081017 http://dx.doi.org/10.1038/nsmb.2164 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Bintu, Lacramioara
Kopaczynska, Marta
Hodges, Courtney
Lubkowska, Lucyna
Kashlev, Mikhail
Bustamante, Carlos
The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title_full The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title_fullStr The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title_full_unstemmed The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title_short The elongation rate of RNA polymerase determines the fate of transcribed nucleosomes
title_sort elongation rate of rna polymerase determines the fate of transcribed nucleosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279329/
https://www.ncbi.nlm.nih.gov/pubmed/22081017
http://dx.doi.org/10.1038/nsmb.2164
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