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Bioactive potential of Streptomyces against fish and shellfish pathogens
BACKGROUND AND OBJECTIVES: In the present study, isolation of Streptomyces associated with marine sponges and its bioactive potential against fish and shellfish pathogens were assessed. The Streptomyces sp. were isolated from the marine sponges namely Callyspongia diffusa, Mycale mytilorum, Tedania...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279779/ https://www.ncbi.nlm.nih.gov/pubmed/22347566 |
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author | Selvakumar, D Arun, K Suguna, S Kumar, D Dhevendaran, K |
author_facet | Selvakumar, D Arun, K Suguna, S Kumar, D Dhevendaran, K |
author_sort | Selvakumar, D |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: In the present study, isolation of Streptomyces associated with marine sponges and its bioactive potential against fish and shellfish pathogens were assessed. The Streptomyces sp. were isolated from the marine sponges namely Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis collected from Vizhinjam port, situated in the South-West coast of India. MATERIALS AND METHODS: The Streptomyces associated with marine sponges were isolated using specific ISP media. The isolates of Streptomyces were characterized for their colony characteristics, morphological properties, physiological and biochemical properties and were tentatively identified. The strains were cultivated on a lab scale level as shake-flask cultures and the crude extracts of the bioactive compounds obtained with ethyl acetate were screened biologically and chemically. By biological screening, the extracts were analyzed for their activity against fish and shellfish pathogens namely Aeromonas hydrophila, Serratia sp. and Vibrio spp, using the disk and agar-well diffusion bioassay method, while by chemical screening the crude culture extracts were analyzed by TLC and UV–Vis spectrophotometer. RESULTS: Ninety-four isolates were found to be associated with marine sponges, among them only seven strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological and biochemical characteristics suggested that these strains belonged to the genus Streptomyces. The initial screening of the isolates by spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In-vitro screening of the submerge culture extracts showed positive inhibition against the fish and shellfish pathogens namely Aeromonas hydrophila, Serratia sp. and Vibrio spp. The screening of bioactive compounds confirmed the production of polyene substances by UV spectrum, which resulted in absorbance peaks ranging from 225 to 245 nm and TLC analysis yielded R(f) values ranging from 0.40 to 0.78. CONCLUSION: The results suggest that the seven Streptomyces strains isolated from marine sponges produce potential antibacterial compounds against fish and shellfish pathogens. |
format | Online Article Text |
id | pubmed-3279779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-32797792012-02-16 Bioactive potential of Streptomyces against fish and shellfish pathogens Selvakumar, D Arun, K Suguna, S Kumar, D Dhevendaran, K Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: In the present study, isolation of Streptomyces associated with marine sponges and its bioactive potential against fish and shellfish pathogens were assessed. The Streptomyces sp. were isolated from the marine sponges namely Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis collected from Vizhinjam port, situated in the South-West coast of India. MATERIALS AND METHODS: The Streptomyces associated with marine sponges were isolated using specific ISP media. The isolates of Streptomyces were characterized for their colony characteristics, morphological properties, physiological and biochemical properties and were tentatively identified. The strains were cultivated on a lab scale level as shake-flask cultures and the crude extracts of the bioactive compounds obtained with ethyl acetate were screened biologically and chemically. By biological screening, the extracts were analyzed for their activity against fish and shellfish pathogens namely Aeromonas hydrophila, Serratia sp. and Vibrio spp, using the disk and agar-well diffusion bioassay method, while by chemical screening the crude culture extracts were analyzed by TLC and UV–Vis spectrophotometer. RESULTS: Ninety-four isolates were found to be associated with marine sponges, among them only seven strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological and biochemical characteristics suggested that these strains belonged to the genus Streptomyces. The initial screening of the isolates by spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In-vitro screening of the submerge culture extracts showed positive inhibition against the fish and shellfish pathogens namely Aeromonas hydrophila, Serratia sp. and Vibrio spp. The screening of bioactive compounds confirmed the production of polyene substances by UV spectrum, which resulted in absorbance peaks ranging from 225 to 245 nm and TLC analysis yielded R(f) values ranging from 0.40 to 0.78. CONCLUSION: The results suggest that the seven Streptomyces strains isolated from marine sponges produce potential antibacterial compounds against fish and shellfish pathogens. Tehran University of Medical Sciences 2010-09 /pmc/articles/PMC3279779/ /pubmed/22347566 Text en © 2010 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Selvakumar, D Arun, K Suguna, S Kumar, D Dhevendaran, K Bioactive potential of Streptomyces against fish and shellfish pathogens |
title | Bioactive potential of Streptomyces against fish and shellfish pathogens |
title_full | Bioactive potential of Streptomyces against fish and shellfish pathogens |
title_fullStr | Bioactive potential of Streptomyces against fish and shellfish pathogens |
title_full_unstemmed | Bioactive potential of Streptomyces against fish and shellfish pathogens |
title_short | Bioactive potential of Streptomyces against fish and shellfish pathogens |
title_sort | bioactive potential of streptomyces against fish and shellfish pathogens |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279779/ https://www.ncbi.nlm.nih.gov/pubmed/22347566 |
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