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Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium

BACKGROUND AND OBJECTIVES: Glycoprotein 96 is the primary chaperone of the endoplasmic reticulum. Immunization with it induced potent Cytotoxic T lymphocyte responses to intracellular bacteria. S. typhimurium is a facultative intracellular bacterium and acquired resistance against this bacterium mai...

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Autores principales: Hosseini Jazani, N, Karimzad, M, Shahabi, S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279793/
https://www.ncbi.nlm.nih.gov/pubmed/22347568
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author Hosseini Jazani, N
Karimzad, M
Shahabi, S
author_facet Hosseini Jazani, N
Karimzad, M
Shahabi, S
author_sort Hosseini Jazani, N
collection PubMed
description BACKGROUND AND OBJECTIVES: Glycoprotein 96 is the primary chaperone of the endoplasmic reticulum. Immunization with it induced potent Cytotoxic T lymphocyte responses to intracellular bacteria. S. typhimurium is a facultative intracellular bacterium and acquired resistance against this bacterium mainly depends on activity of Cytotoxic T cells. This study aimed to evaluate the capacity of Glycoprotein 96 rich lysate as a vaccine candidate to induce a protective immune response in mice against a lethal dose challenge with Salmonella typhimurium. MATERIALS AND METHODS: Mice were infected with S. typhimurium. Then their spleens and livers were harvested and homogenized and the protein content of whole crude lysate was enriched using ammonium sulfate precipitation. SDS-polyacrylamide gel electrophoresis transfer method was used for enrichment of the protein from crude sample. Immunoblotting was conducted to detect Glycoprotein 96. Isoelectric point was achieved through the use of isoelectric focusing. PBS and whole crude lysate (from uninfected and infected mice) were injected to mice of test group, mice of control-1 group and mice of control-2 group, respectively, on days 0 and 14. Twenty-one days after the last immunization, the LD50 and bacterial loads of livers and spleens were determined. RESULTS AND CONCLUSION: Immunization with Glycoprotein 96 rich lysate isolated from livers and spleens of S. typhimuriuminfected mice induced protection against infection by S. typhimurium. Also, the bacterial burden of livers and spleens in mice that received gp96 rich lysate significantly decreased when compared to that of mice in the control groups.
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spelling pubmed-32797932012-02-16 Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium Hosseini Jazani, N Karimzad, M Shahabi, S Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Glycoprotein 96 is the primary chaperone of the endoplasmic reticulum. Immunization with it induced potent Cytotoxic T lymphocyte responses to intracellular bacteria. S. typhimurium is a facultative intracellular bacterium and acquired resistance against this bacterium mainly depends on activity of Cytotoxic T cells. This study aimed to evaluate the capacity of Glycoprotein 96 rich lysate as a vaccine candidate to induce a protective immune response in mice against a lethal dose challenge with Salmonella typhimurium. MATERIALS AND METHODS: Mice were infected with S. typhimurium. Then their spleens and livers were harvested and homogenized and the protein content of whole crude lysate was enriched using ammonium sulfate precipitation. SDS-polyacrylamide gel electrophoresis transfer method was used for enrichment of the protein from crude sample. Immunoblotting was conducted to detect Glycoprotein 96. Isoelectric point was achieved through the use of isoelectric focusing. PBS and whole crude lysate (from uninfected and infected mice) were injected to mice of test group, mice of control-1 group and mice of control-2 group, respectively, on days 0 and 14. Twenty-one days after the last immunization, the LD50 and bacterial loads of livers and spleens were determined. RESULTS AND CONCLUSION: Immunization with Glycoprotein 96 rich lysate isolated from livers and spleens of S. typhimuriuminfected mice induced protection against infection by S. typhimurium. Also, the bacterial burden of livers and spleens in mice that received gp96 rich lysate significantly decreased when compared to that of mice in the control groups. Tehran University of Medical Sciences 2010-12 /pmc/articles/PMC3279793/ /pubmed/22347568 Text en © 2010 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Hosseini Jazani, N
Karimzad, M
Shahabi, S
Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title_full Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title_fullStr Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title_full_unstemmed Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title_short Gp96 rich lysate as a vaccine candidate against infection with Salmonella typhimurium
title_sort gp96 rich lysate as a vaccine candidate against infection with salmonella typhimurium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279793/
https://www.ncbi.nlm.nih.gov/pubmed/22347568
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