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Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis

BACKGROUND: Considering that ELISA method presently is the test of choice for diagnosis of fasciolosis, the present study was undertaken to evaluate the maximum validity of coated plates at different temperatures and different times during one year of evaluation. METHODS: Serum samples of patients i...

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Autores principales: Rokni, MB, Aryaeipour, M, Koosha, S, Rahimi, MT
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279827/
https://www.ncbi.nlm.nih.gov/pubmed/22347234
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author Rokni, MB
Aryaeipour, M
Koosha, S
Rahimi, MT
author_facet Rokni, MB
Aryaeipour, M
Koosha, S
Rahimi, MT
author_sort Rokni, MB
collection PubMed
description BACKGROUND: Considering that ELISA method presently is the test of choice for diagnosis of fasciolosis, the present study was undertaken to evaluate the maximum validity of coated plates at different temperatures and different times during one year of evaluation. METHODS: Serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5), toxocariasis (n=5), and negative control sera (n=5) were examined. Two series of plates were considered. The first series were coated with Fasciola homogenate Ag 12 ug/ml, and after some steps were blocked with gelatin and preserved at different temperatures as −80°C, −20°C, −4°C and +4°C. The 2(nd) series were treated under the same criteria but were not blocked with gelatin. Each series were examined by ELISA test from 1(st) month to 12(th) month. Sera with 1:125 dilution, and peroxidase-conjugated goat anti-human IgG diluted 1:10000 were considered optimum. RESULTS: To ease reporting the results and due to many similarities only results related to 1(st), 6(th) and 12(th) months were analyzed and sensitivity, specificity plus cut-off were determined for each series separately. CONCLUSION: Preserving the coated plates, while unblocked at −80°C for 6–8 months is pertinent and functional and in that case, we can be sure the best out put would be applicable.
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spelling pubmed-32798272012-02-16 Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis Rokni, MB Aryaeipour, M Koosha, S Rahimi, MT Iran J Parasitol Original Article BACKGROUND: Considering that ELISA method presently is the test of choice for diagnosis of fasciolosis, the present study was undertaken to evaluate the maximum validity of coated plates at different temperatures and different times during one year of evaluation. METHODS: Serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5), toxocariasis (n=5), and negative control sera (n=5) were examined. Two series of plates were considered. The first series were coated with Fasciola homogenate Ag 12 ug/ml, and after some steps were blocked with gelatin and preserved at different temperatures as −80°C, −20°C, −4°C and +4°C. The 2(nd) series were treated under the same criteria but were not blocked with gelatin. Each series were examined by ELISA test from 1(st) month to 12(th) month. Sera with 1:125 dilution, and peroxidase-conjugated goat anti-human IgG diluted 1:10000 were considered optimum. RESULTS: To ease reporting the results and due to many similarities only results related to 1(st), 6(th) and 12(th) months were analyzed and sensitivity, specificity plus cut-off were determined for each series separately. CONCLUSION: Preserving the coated plates, while unblocked at −80°C for 6–8 months is pertinent and functional and in that case, we can be sure the best out put would be applicable. Tehran University of Medical Sciences 2010-03 /pmc/articles/PMC3279827/ /pubmed/22347234 Text en © 2010 Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rokni, MB
Aryaeipour, M
Koosha, S
Rahimi, MT
Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title_full Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title_fullStr Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title_full_unstemmed Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title_short Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
title_sort evaluation of the stability of coated plates with antigen at different temperatures and times by elisa test to diagnose fasciolosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279827/
https://www.ncbi.nlm.nih.gov/pubmed/22347234
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